RNAseq expression analysis of resistant and susceptible mice after influenza A virus infection identifies novel genes associated with virus replication and important for host resistance to infection

2.50
Hdl Handle:
http://hdl.handle.net/10033/620950
Title:
RNAseq expression analysis of resistant and susceptible mice after influenza A virus infection identifies novel genes associated with virus replication and important for host resistance to infection
Authors:
Wilk, Esther; Pandey, Ashutosh K; Leist, Sarah R; Hatesuer, Bastian; Preusse, Matthias; Pommerenke, Claudia; Wang, Junxi; Schughart, Klaus
Abstract:
Abstract Background The host response to influenza A infections is strongly influenced by host genetic factors. Animal models of genetically diverse mouse strains are well suited to identify host genes involved in severe pathology, viral replication and immune responses. Here, we have utilized a dual RNAseq approach that allowed us to investigate both viral and host gene expression in the same individual mouse after H1N1 infection. Results We performed a detailed expression analysis to identify (i) correlations between changes in expression of host and virus genes, (ii) host genes involved in viral replication, and (iii) genes showing differential expression between two mouse strains that strongly differ in resistance to influenza infections. These genes may be key players involved in regulating the differences in pathogenesis and host defense mechanisms after influenza A infections. Expression levels of influenza segments correlated well with the viral load and may thus be used as surrogates for conventional viral load measurements. Furthermore, we investigated the functional role of two genes, Reg3g and Irf7, in knock-out mice and found that deletion of the Irf7 gene renders the host highly susceptible to H1N1 infection. Conclusions Using RNAseq analysis we identified novel genes important for viral replication or the host defense. This study adds further important knowledge to host-pathogen-interactions and suggests additional candidates that are crucial for host susceptibility or survival during influenza A infections.
Citation:
BMC Genomics. 2015 Sep 02;16(1):655
Issue Date:
2-Sep-2015
URI:
http://dx.doi.org/10.1186/s12864-015-1867-8; http://hdl.handle.net/10033/620950
Type:
Journal Article
Appears in Collections:
publications of the department infection genetics (INFG)

Full metadata record

DC FieldValue Language
dc.contributor.authorWilk, Estheren
dc.contributor.authorPandey, Ashutosh Ken
dc.contributor.authorLeist, Sarah Ren
dc.contributor.authorHatesuer, Bastianen
dc.contributor.authorPreusse, Matthiasen
dc.contributor.authorPommerenke, Claudiaen
dc.contributor.authorWang, Junxien
dc.contributor.authorSchughart, Klausen
dc.date.accessioned2017-06-15T09:02:14Z-
dc.date.available2017-06-15T09:02:14Z-
dc.date.issued2015-09-02en
dc.identifier.citationBMC Genomics. 2015 Sep 02;16(1):655en
dc.identifier.urihttp://dx.doi.org/10.1186/s12864-015-1867-8en
dc.identifier.urihttp://hdl.handle.net/10033/620950-
dc.description.abstractAbstract Background The host response to influenza A infections is strongly influenced by host genetic factors. Animal models of genetically diverse mouse strains are well suited to identify host genes involved in severe pathology, viral replication and immune responses. Here, we have utilized a dual RNAseq approach that allowed us to investigate both viral and host gene expression in the same individual mouse after H1N1 infection. Results We performed a detailed expression analysis to identify (i) correlations between changes in expression of host and virus genes, (ii) host genes involved in viral replication, and (iii) genes showing differential expression between two mouse strains that strongly differ in resistance to influenza infections. These genes may be key players involved in regulating the differences in pathogenesis and host defense mechanisms after influenza A infections. Expression levels of influenza segments correlated well with the viral load and may thus be used as surrogates for conventional viral load measurements. Furthermore, we investigated the functional role of two genes, Reg3g and Irf7, in knock-out mice and found that deletion of the Irf7 gene renders the host highly susceptible to H1N1 infection. Conclusions Using RNAseq analysis we identified novel genes important for viral replication or the host defense. This study adds further important knowledge to host-pathogen-interactions and suggests additional candidates that are crucial for host susceptibility or survival during influenza A infections.en
dc.titleRNAseq expression analysis of resistant and susceptible mice after influenza A virus infection identifies novel genes associated with virus replication and important for host resistance to infectionen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderWilk et al.en
dc.date.updated2015-09-04T08:24:20Zen
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