2.50
Hdl Handle:
http://hdl.handle.net/10033/620953
Title:
Sequencing and Characterization of Pseudomonas aeruginosa phage JG004
Authors:
Garbe, Julia; Bunk, Boyke; Rohde, Manfred; Schobert, Max
Abstract:
Abstract Background Phages could be an important alternative to antibiotics, especially for treatment of multiresistant bacteria as e.g. Pseudomonas aeruginosa. For an effective use of bacteriophages as antimicrobial agents, it is important to understand phage biology but also genes of the bacterial host essential for phage infection. Results We isolated and characterized a lytic Pseudomonas aeruginosa phage, named JG004, and sequenced its genome. Phage JG004 is a lipopolysaccharide specific broad-host-range phage of the Myoviridae phage family. The genome of phage JG004 encodes twelve tRNAs and is highly related to the PAK-P1 phage genome. To investigate phage biology and phage-host interactions, we used transposon mutagenesis of the P. aeruginosa host and identified P. aeruginosa genes, which are essential for phage infection. Analysis of the respective P. aeruginosa mutants revealed several characteristics, such as host receptor and possible spermidine-dependance of phage JG004. Conclusions Whole genome sequencing of phage JG004 in combination with identification of P. aeruginosa host genes essential for infection, allowed insights into JG004 biology, revealed possible resistance mechanisms of the host bacterium such as mutations in LPS and spermidine biosynthesis and can also be used to characterize unknown gene products in P. aeruginosa.
Citation:
BMC Microbiology. 2011 May 14;11(1):102
Issue Date:
14-May-2011
URI:
http://dx.doi.org/10.1186/1471-2180-11-102; http://hdl.handle.net/10033/620953
Type:
Journal Article
Appears in Collections:
publications of the central unit for microscopy (ZEIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorGarbe, Juliaen
dc.contributor.authorBunk, Boykeen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorSchobert, Maxen
dc.date.accessioned2017-06-15T10:33:07Z-
dc.date.available2017-06-15T10:33:07Z-
dc.date.issued2011-05-14en
dc.identifier.citationBMC Microbiology. 2011 May 14;11(1):102en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-11-102en
dc.identifier.urihttp://hdl.handle.net/10033/620953-
dc.description.abstractAbstract Background Phages could be an important alternative to antibiotics, especially for treatment of multiresistant bacteria as e.g. Pseudomonas aeruginosa. For an effective use of bacteriophages as antimicrobial agents, it is important to understand phage biology but also genes of the bacterial host essential for phage infection. Results We isolated and characterized a lytic Pseudomonas aeruginosa phage, named JG004, and sequenced its genome. Phage JG004 is a lipopolysaccharide specific broad-host-range phage of the Myoviridae phage family. The genome of phage JG004 encodes twelve tRNAs and is highly related to the PAK-P1 phage genome. To investigate phage biology and phage-host interactions, we used transposon mutagenesis of the P. aeruginosa host and identified P. aeruginosa genes, which are essential for phage infection. Analysis of the respective P. aeruginosa mutants revealed several characteristics, such as host receptor and possible spermidine-dependance of phage JG004. Conclusions Whole genome sequencing of phage JG004 in combination with identification of P. aeruginosa host genes essential for infection, allowed insights into JG004 biology, revealed possible resistance mechanisms of the host bacterium such as mutations in LPS and spermidine biosynthesis and can also be used to characterize unknown gene products in P. aeruginosa.en
dc.titleSequencing and Characterization of Pseudomonas aeruginosa phage JG004en
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderGarbe et al; licensee BioMed Central Ltd.en
dc.date.updated2015-09-04T08:25:12Zen
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