2.50
Hdl Handle:
http://hdl.handle.net/10033/620959
Title:
An oral multispecies biofilm model for high content screening applications.
Authors:
Kommerein, Nadine; Stumpp, Sascha N; Müsken, Mathias; Ehlert, Nina; Winkel, Andreas; Häussler, Susanne; Behrens, Peter; Buettner, Falk F R; Stiesch, Meike
Abstract:
Peri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. The bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. A promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. A reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefore of major importance. In the present study, we developed a highly reproducible in vitro four-species biofilm model consisting of the highly relevant oral bacterial species Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis. The application of live/dead staining, quantitative real time PCR (qRT-PCR), scanning electron microscopy (SEM) and urea-NaCl fluorescence in situ hybridization (urea-NaCl-FISH) revealed that the four-species biofilm community is robust in terms of biovolume, live/dead distribution and individual species distribution over time. The biofilm community is dominated by S. oralis, followed by V. dispar, A. naeslundii and P. gingivalis. The percentage distribution in this model closely reflects the situation in early native plaques and is therefore well suited as an in vitro model test system. Furthermore, despite its nearly native composition, the multispecies model does not depend on nutrient additives, such as native human saliva or serum, and is an inexpensive, easy to handle and highly reproducible alternative to the available model systems. The 96-well plate format enables high content screening for optimized implant surfaces impeding biofilm formation or the testing of multiple antimicrobial treatment strategies to fight multispecies biofilm infections, both exemplary proven in the manuscript.
Affiliation:
Helmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany.
Citation:
An oral multispecies biofilm model for high content screening applications. 2017, 12 (3):e0173973 PLoS ONE
Journal:
PloS one
Issue Date:
2017
URI:
http://hdl.handle.net/10033/620959
DOI:
10.1371/journal.pone.0173973
PubMed ID:
28296966
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
publications of the departmentment of molecular bacteriology(MOBA)

Full metadata record

DC FieldValue Language
dc.contributor.authorKommerein, Nadineen
dc.contributor.authorStumpp, Sascha Nen
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorEhlert, Ninaen
dc.contributor.authorWinkel, Andreasen
dc.contributor.authorHäussler, Susanneen
dc.contributor.authorBehrens, Peteren
dc.contributor.authorBuettner, Falk F Ren
dc.contributor.authorStiesch, Meikeen
dc.date.accessioned2017-06-19T13:17:48Z-
dc.date.available2017-06-19T13:17:48Z-
dc.date.issued2017-
dc.identifier.citationAn oral multispecies biofilm model for high content screening applications. 2017, 12 (3):e0173973 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid28296966-
dc.identifier.doi10.1371/journal.pone.0173973-
dc.identifier.urihttp://hdl.handle.net/10033/620959-
dc.description.abstractPeri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. The bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. A promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. A reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefore of major importance. In the present study, we developed a highly reproducible in vitro four-species biofilm model consisting of the highly relevant oral bacterial species Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis. The application of live/dead staining, quantitative real time PCR (qRT-PCR), scanning electron microscopy (SEM) and urea-NaCl fluorescence in situ hybridization (urea-NaCl-FISH) revealed that the four-species biofilm community is robust in terms of biovolume, live/dead distribution and individual species distribution over time. The biofilm community is dominated by S. oralis, followed by V. dispar, A. naeslundii and P. gingivalis. The percentage distribution in this model closely reflects the situation in early native plaques and is therefore well suited as an in vitro model test system. Furthermore, despite its nearly native composition, the multispecies model does not depend on nutrient additives, such as native human saliva or serum, and is an inexpensive, easy to handle and highly reproducible alternative to the available model systems. The 96-well plate format enables high content screening for optimized implant surfaces impeding biofilm formation or the testing of multiple antimicrobial treatment strategies to fight multispecies biofilm infections, both exemplary proven in the manuscript.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAn oral multispecies biofilm model for high content screening applications.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr.7, 38124 Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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