Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture.

2.50
Hdl Handle:
http://hdl.handle.net/10033/621373
Title:
Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture.
Authors:
Olmer, Ruth; Engels, Lena; Usman, Abdulai; Menke, Sandra; Malik, Muhammad Nasir Hayat; Pessler, Frank; Göhring, Gudrun; Bornhorst, Dorothee; Bolten, Svenja; Abdelilah-Seyfried, Salim; Scheper, Thomas; Kempf, Henning; Zweigerdt, Robert; Martin, Ulrich
Abstract:
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
Affiliation:
TWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
Citation:
Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture. 2018, 10 (5):1657-1672 Stem Cell Reports
Journal:
Stem cell reports
Issue Date:
8-May-2018
URI:
http://hdl.handle.net/10033/621373
DOI:
10.1016/j.stemcr.2018.03.017
PubMed ID:
29681541
Type:
Article
Language:
en
ISSN:
2213-6711
Appears in Collections:
publications of the research group biomarker in infection and immunity [[TC] BIOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorOlmer, Ruthen
dc.contributor.authorEngels, Lenaen
dc.contributor.authorUsman, Abdulaien
dc.contributor.authorMenke, Sandraen
dc.contributor.authorMalik, Muhammad Nasir Hayaten
dc.contributor.authorPessler, Franken
dc.contributor.authorGöhring, Gudrunen
dc.contributor.authorBornhorst, Dorotheeen
dc.contributor.authorBolten, Svenjaen
dc.contributor.authorAbdelilah-Seyfried, Salimen
dc.contributor.authorScheper, Thomasen
dc.contributor.authorKempf, Henningen
dc.contributor.authorZweigerdt, Roberten
dc.contributor.authorMartin, Ulrichen
dc.date.accessioned2018-05-16T12:56:46Z-
dc.date.available2018-05-16T12:56:46Z-
dc.date.issued2018-05-08-
dc.identifier.citationDifferentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture. 2018, 10 (5):1657-1672 Stem Cell Reportsen
dc.identifier.issn2213-6711-
dc.identifier.pmid29681541-
dc.identifier.doi10.1016/j.stemcr.2018.03.017-
dc.identifier.urihttp://hdl.handle.net/10033/621373-
dc.description.abstractEndothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.en
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDifferentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture.en
dc.typeArticleen
dc.contributor.departmentTWINCORE, Zentrum für experimentelle und klinischeInfektionsforschung GmbH, Feodor-Lynen-Str. 7, 30625 Hannover, Germany.en
dc.identifier.journalStem cell reportsen

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