Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.

2.50
Hdl Handle:
http://hdl.handle.net/10033/71233
Title:
Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.
Authors:
de Beer, Dalene; Jerz, Gerold; Joubert, Elizabeth; Wray, Victor; Winterhalter, Peter
Abstract:
Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.
Affiliation:
ARC Infruitec-Nietvoorbij, Stellenbosch, South Africa. dbeerd@arc.agric.za
Citation:
Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography. 2009, 1216 (19):4282-9 J Chromatogr A
Journal:
Journal of chromatography. A
Issue Date:
8-May-2009
URI:
http://hdl.handle.net/10033/71233
DOI:
10.1016/j.chroma.2009.02.056
PubMed ID:
19272608
Type:
Article
Language:
en
ISSN:
1873-3778
Appears in Collections:
Publications from Division of Molekulare Struktur Biologie (MOSB)

Full metadata record

DC FieldValue Language
dc.contributor.authorde Beer, Dalene-
dc.contributor.authorJerz, Gerold-
dc.contributor.authorJoubert, Elizabeth-
dc.contributor.authorWray, Victor-
dc.contributor.authorWinterhalter, Peter-
dc.date.accessioned2009-06-23T08:26:46Z-
dc.date.available2009-06-23T08:26:46Z-
dc.date.issued2009-05-08-
dc.identifier.citationIsolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography. 2009, 1216 (19):4282-9 J Chromatogr Aen
dc.identifier.issn1873-3778-
dc.identifier.pmid19272608-
dc.identifier.doi10.1016/j.chroma.2009.02.056-
dc.identifier.urihttp://hdl.handle.net/10033/71233-
dc.description.abstractIsomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.en
dc.language.isoenen
dc.titleIsolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.en
dc.typeArticleen
dc.contributor.departmentARC Infruitec-Nietvoorbij, Stellenbosch, South Africa. dbeerd@arc.agric.zaen
dc.identifier.journalJournal of chromatography. Aen
This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.