2.50
HDL Handle:
http://hdl.handle.net/10033/76653
Title:
Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties.
Authors:
Qiao, Junhua; Oumard, André; Wegloehner, Wolfgang; Bode, Juergen
Abstract:
Site-specific recombinases have revolutionized the systematic generation of transgenic cell lines and embryonic stem cells/animals and will ultimately also reveal their potential in the genetic modification of induced pluripotent stem cells. Introduced in 1994, our Flp recombinase-mediated cassette exchange strategy permits the exchange of a target cassette for a cassette with the gene of interest, introduced as a part of an exchange vector. The process is "clean" in the sense that it does not co-introduce prokaryotic vector parts; neither does it leave behind a selection marker. Stringent selection principles provide master cell lines permitting subsequent recombinase-mediated cassette exchange cycles in the absence of a drug selection and with a considerable efficiency (approximately 10%). Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype.
Affiliation:
Department of Molecular Biotechnology/Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties. 2009, 390 (4):579-94 J. Mol. Biol.
Journal:
Journal of molecular biology
Issue Date:
24-Jul-2009
URI:
http://hdl.handle.net/10033/76653
DOI:
10.1016/j.jmb.2009.05.012
PubMed ID:
19447116
Type:
Article
Language:
en
ISSN:
1089-8638
Appears in Collections:
Publications of RG Epigenetic Regulation Mechanisms (EPI)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorQiao, Junhua-
dc.contributor.authorOumard, André-
dc.contributor.authorWegloehner, Wolfgang-
dc.contributor.authorBode, Juergen-
dc.date.accessioned2009-08-07T09:05:59Z-
dc.date.available2009-08-07T09:05:59Z-
dc.date.issued2009-07-24-
dc.identifier.citationNovel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties. 2009, 390 (4):579-94 J. Mol. Biol.en
dc.identifier.issn1089-8638-
dc.identifier.pmid19447116-
dc.identifier.doi10.1016/j.jmb.2009.05.012-
dc.identifier.urihttp://hdl.handle.net/10033/76653-
dc.description.abstractSite-specific recombinases have revolutionized the systematic generation of transgenic cell lines and embryonic stem cells/animals and will ultimately also reveal their potential in the genetic modification of induced pluripotent stem cells. Introduced in 1994, our Flp recombinase-mediated cassette exchange strategy permits the exchange of a target cassette for a cassette with the gene of interest, introduced as a part of an exchange vector. The process is "clean" in the sense that it does not co-introduce prokaryotic vector parts; neither does it leave behind a selection marker. Stringent selection principles provide master cell lines permitting subsequent recombinase-mediated cassette exchange cycles in the absence of a drug selection and with a considerable efficiency (approximately 10%). Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype.en
dc.language.isoenen
dc.titleNovel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biotechnology/Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalJournal of molecular biologyen
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