Identification of cardiac malformations in mice lacking Ptdsr using a novel high-throughput magnetic resonance imaging technique

2.50
Hdl Handle:
http://hdl.handle.net/10033/8684
Title:
Identification of cardiac malformations in mice lacking Ptdsr using a novel high-throughput magnetic resonance imaging technique
Authors:
Schneider, Jürgen E; Böse, Jens; Bamforth, Simon D; Gruber, Achim D; Broadbent, Carol; Clarke, Kieran; Neubauer, Stefan; Lengeling, Andreas; Bhattacharya, Shoumo
Abstract:
Background Congenital heart defects are the leading non-infectious cause of death in children. Genetic studies in the mouse have been crucial to uncover new genes and signaling pathways associated with heart development and congenital heart disease. The identification of murine models of congenital cardiac malformations in high-throughput mutagenesis screens and in gene-targeted models is hindered by the opacity of the mouse embryo. Results We developed and optimized a novel method for high-throughput multi-embryo magnetic resonance imaging (MRI). Using this approach we identified cardiac malformations in phosphatidylserine receptor (Ptdsr) deficient embryos. These included ventricular septal defects, double-outlet right ventricle, and hypoplasia of the pulmonary artery and thymus. These results indicate that Ptdsr plays a key role in cardiac development. Conclusions Our novel multi-embryo MRI technique enables high-throughput identification of murine models for human congenital cardiopulmonary malformations at high spatial resolution. The technique can be easily adapted for mouse mutagenesis screens and, thus provides an important new tool for identifying new mouse models for human congenital heart diseases.
Citation:
BMC Developmental Biology 2004 4:16
Publisher:
BioMed Central
Issue Date:
22-Dec-2004
URI:
http://hdl.handle.net/10033/8684
DOI:
10.1186/1471-213X-4-16
PubMed ID:
15615595
PubMed Central ID:
545075
Additional Links:
http://www.biomedcentral.com/1471-213X/4/16; http://creativecommons.org/licenses/by/2.0
Language:
en_US
ISSN:
1471-213X
Appears in Collections:
publications of the department infection genetics (INFG)

Full metadata record

DC FieldValue Language
dc.contributor.authorSchneider, Jürgen Een_US
dc.contributor.authorBöse, Jensen_US
dc.contributor.authorBamforth, Simon Den_US
dc.contributor.authorGruber, Achim Den_US
dc.contributor.authorBroadbent, Carolen_US
dc.contributor.authorClarke, Kieranen_US
dc.contributor.authorNeubauer, Stefanen_US
dc.contributor.authorLengeling, Andreasen_US
dc.contributor.authorBhattacharya, Shoumoen_US
dc.date.accessioned2007-02-21T08:21:50Z-
dc.date.available2004-12-22en_US
dc.date.available2007-02-21T08:21:50Z-
dc.date.issued2004-12-22en_US
dc.identifier.citationBMC Developmental Biology 2004 4:16en_US
dc.identifier.issn1471-213Xen_US
dc.identifier.pmid15615595en_US
dc.identifier.doi10.1186/1471-213X-4-16en_US
dc.identifier.urihttp://hdl.handle.net/10033/8684-
dc.description.abstractBackground Congenital heart defects are the leading non-infectious cause of death in children. Genetic studies in the mouse have been crucial to uncover new genes and signaling pathways associated with heart development and congenital heart disease. The identification of murine models of congenital cardiac malformations in high-throughput mutagenesis screens and in gene-targeted models is hindered by the opacity of the mouse embryo. Results We developed and optimized a novel method for high-throughput multi-embryo magnetic resonance imaging (MRI). Using this approach we identified cardiac malformations in phosphatidylserine receptor (Ptdsr) deficient embryos. These included ventricular septal defects, double-outlet right ventricle, and hypoplasia of the pulmonary artery and thymus. These results indicate that Ptdsr plays a key role in cardiac development. Conclusions Our novel multi-embryo MRI technique enables high-throughput identification of murine models for human congenital cardiopulmonary malformations at high spatial resolution. The technique can be easily adapted for mouse mutagenesis screens and, thus provides an important new tool for identifying new mouse models for human congenital heart diseases.en_US
dc.language.isoen_US-
dc.publisherBioMed Centralen_US
dc.relation.urlhttp://www.biomedcentral.com/1471-213X/4/16en_US
dc.relation.urlhttp://creativecommons.org/licenses/by/2.0en_US
dc.rightsCopyright © 2004 Schneider et al; licensee BioMed Central Ltd.en_US
dc.titleIdentification of cardiac malformations in mice lacking Ptdsr using a novel high-throughput magnetic resonance imaging techniqueen_US
dc.identifier.pmcid545075en_US
dc.format.digYES-

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