Cationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopy

2.50
HDL Handle:
http://hdl.handle.net/10033/8763
Title:
Cationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopy
Authors:
Lünsdorf, Heinrich; Kristen, Ingeborg; Barth, Elke
Abstract:
Background Synthesis of cationic hydrous thorium dioxide colloids (ca. 1.0 to 1.7 nm) has been originally described by Müller [22] and Groot [11] and these have been used by Groot to stain acidic glucosaminoglycans for ultrastructure research of different tissues by conventional transmission electron microscopy. Results Synthesis of colloidal thorium dioxide has been modified and its use as a suitable stain of acidic mucopolysaccharides and other anionic biopolymers from bacteria, either as whole mount preparations or as preembedment labels, is described. The differences in stain behavior relative to commonly used rutheniumred-lysine and Alcian Blue™ electron dense acidic stains has been investigated and its use is exemplified for Pseudomonas aeruginosa adjacent cell wall biopolymers. For the first time thorificated biopolymers, i.e. bacterial outer cell wall layers, have been analysed at the ultrastructural level with electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI), leading to excellent contrast and signal strength for these extracellular biopolymers. Conclusion Application of cationic hydrous ThO2 colloids for tracing acidic groups of the bacterial surface and/or EPS has been shown to be rather effective by transmission electron microscopy. Because of its high electron density and its good diffusibility it stains and outlines electro-negative charges within these biopolymers. In combination with ESI, based on integrated energy-filtered electron microscopy (EFTEM) Th-densities and thus negative charge densities can be discriminated from other elemental densities, especially in environmental samples, such as biofilms.
Citation:
BMC Microbiology 2006 6:59
Publisher:
BioMed Central
Issue Date:
27-Jun-2006
URI:
http://hdl.handle.net/10033/8763
DOI:
10.1186/1471-2180-6-59
PubMed ID:
16803626
PubMed Central ID:
1524781
Additional Links:
http://www.biomedcentral.com/1471-2180/6/59; http://creativecommons.org/licenses/by/2.0
Language:
en_US
ISSN:
1471-2180
Appears in Collections:
Publications of Division of Microbiology (MIK)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorLünsdorf, Heinrichen_US
dc.contributor.authorKristen, Ingeborgen_US
dc.contributor.authorBarth, Elkeen_US
dc.date.accessioned2007-02-22T14:59:18Z-
dc.date.available2006-06-27en_US
dc.date.available2007-02-22T14:59:18Z-
dc.date.issued2006-06-27en_US
dc.identifier.citationBMC Microbiology 2006 6:59en_US
dc.identifier.issn1471-2180en_US
dc.identifier.pmid16803626en_US
dc.identifier.doi10.1186/1471-2180-6-59en_US
dc.identifier.urihttp://hdl.handle.net/10033/8763-
dc.description.abstractBackground Synthesis of cationic hydrous thorium dioxide colloids (ca. 1.0 to 1.7 nm) has been originally described by Müller [22] and Groot [11] and these have been used by Groot to stain acidic glucosaminoglycans for ultrastructure research of different tissues by conventional transmission electron microscopy. Results Synthesis of colloidal thorium dioxide has been modified and its use as a suitable stain of acidic mucopolysaccharides and other anionic biopolymers from bacteria, either as whole mount preparations or as preembedment labels, is described. The differences in stain behavior relative to commonly used rutheniumred-lysine and Alcian Blue™ electron dense acidic stains has been investigated and its use is exemplified for Pseudomonas aeruginosa adjacent cell wall biopolymers. For the first time thorificated biopolymers, i.e. bacterial outer cell wall layers, have been analysed at the ultrastructural level with electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI), leading to excellent contrast and signal strength for these extracellular biopolymers. Conclusion Application of cationic hydrous ThO2 colloids for tracing acidic groups of the bacterial surface and/or EPS has been shown to be rather effective by transmission electron microscopy. Because of its high electron density and its good diffusibility it stains and outlines electro-negative charges within these biopolymers. In combination with ESI, based on integrated energy-filtered electron microscopy (EFTEM) Th-densities and thus negative charge densities can be discriminated from other elemental densities, especially in environmental samples, such as biofilms.en_US
dc.language.isoen_US-
dc.publisherBioMed Centralen_US
dc.relation.urlhttp://www.biomedcentral.com/1471-2180/6/59en_US
dc.relation.urlhttp://creativecommons.org/licenses/by/2.0en_US
dc.rightsCopyright © 2006 Lünsdorf et al; licensee BioMed Central Ltd.en_US
dc.titleCationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopyen_US
dc.identifier.pmcid1524781en_US
dc.format.digYES-

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