Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome.

2.50
HDL Handle:
http://hdl.handle.net/10033/88755
Title:
Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome.
Authors:
Kunze, Markus; Zerlin, Kay F; Retzlaff, Alexander; Pohl, Jens O; Schmidt, Eberhard; Janssen, Dick B; Vilchez-Vargas, Ramiro; Pieper, Dietmar H; Reineke, Walter
Abstract:
Pseudomonas putida GJ31 has been reported to grow on chlorobenzene using a meta-cleavage pathway with chlorocatechol 2,3-dioxygenase (CbzE) as a key enzyme. The CbzE-encoding gene was found to be localized on the 180 kb plasmid pKW1 in a cbzTEXGS cluster, which is flanked by transposases and encodes only a partial (chloro)catechol meta-cleavage pathway comprising ferredoxin reductase, chlorocatechol 2,3-dioxygenase, an unknown protein, 2-hydroxymuconic semialdehyde dehydrogenase and glutathione S-transferase. Downstream of cbzTEXGS are located cbzJ, encoding a novel type of 2-hydroxypent-2,4-dienoate hydratase, and a transposon region highly similar to Tn5501. Upstream of cbzTEXGS, traNEOFG transfer genes were found. The search for gene clusters possibly completing the (chloro)catechol metabolic pathway of GJ31 revealed the presence of two additional catabolic gene clusters on pKW1. The mhpRBCDFETP cluster encodes enzymes for the dissimilation of 2,3-dihydroxyphenylpropionate in a novel arrangement characterized by the absence of a gene encoding 3-(3-hydroxyphenyl)propionate monooxygenase and the presence of a GntR-type regulator, whereas the nahINLOMKJ cluster encodes part of the naphthalene metabolic pathway. Transcription studies supported their possible involvement in chlorobenzene degradation. The upper pathway cluster, comprising genes encoding a chlorobenzene dioxygenase and a chlorobenzene dihydrodiol dehydrogenase, was localized on the chromosome. A high level of transcription in response to chlorobenzene revealed it to be crucial for chlorobenzene degradation. The chlorobenzene degradation pathway in strain GJ31 is thus a mosaic encoded by four gene clusters.
Affiliation:
Bergische Universität Wuppertal, Chemical Microbiology, D-42097 Wuppertal, Germany.
Citation:
Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome. 2009, 155 (Pt 12):4069-83 Microbiology (Reading, Engl.)
Journal:
Microbiology (Reading, England)
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10033/88755
DOI:
10.1099/mic.0.032110-0
PubMed ID:
19744988
Type:
Article
Language:
en
ISSN:
1465-2080
Appears in Collections:
Publications of RG Mikrobielle Interaktionen und Prozesse (MINP)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKunze, Markusen
dc.contributor.authorZerlin, Kay Fen
dc.contributor.authorRetzlaff, Alexanderen
dc.contributor.authorPohl, Jens Oen
dc.contributor.authorSchmidt, Eberharden
dc.contributor.authorJanssen, Dick Ben
dc.contributor.authorVilchez-Vargas, Ramiroen
dc.contributor.authorPieper, Dietmar Hen
dc.contributor.authorReineke, Walteren
dc.date.accessioned2010-01-05T13:36:37Z-
dc.date.available2010-01-05T13:36:37Z-
dc.date.issued2009-12-
dc.identifier.citationDegradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome. 2009, 155 (Pt 12):4069-83 Microbiology (Reading, Engl.)en
dc.identifier.issn1465-2080-
dc.identifier.pmid19744988-
dc.identifier.doi10.1099/mic.0.032110-0-
dc.identifier.urihttp://hdl.handle.net/10033/88755-
dc.description.abstractPseudomonas putida GJ31 has been reported to grow on chlorobenzene using a meta-cleavage pathway with chlorocatechol 2,3-dioxygenase (CbzE) as a key enzyme. The CbzE-encoding gene was found to be localized on the 180 kb plasmid pKW1 in a cbzTEXGS cluster, which is flanked by transposases and encodes only a partial (chloro)catechol meta-cleavage pathway comprising ferredoxin reductase, chlorocatechol 2,3-dioxygenase, an unknown protein, 2-hydroxymuconic semialdehyde dehydrogenase and glutathione S-transferase. Downstream of cbzTEXGS are located cbzJ, encoding a novel type of 2-hydroxypent-2,4-dienoate hydratase, and a transposon region highly similar to Tn5501. Upstream of cbzTEXGS, traNEOFG transfer genes were found. The search for gene clusters possibly completing the (chloro)catechol metabolic pathway of GJ31 revealed the presence of two additional catabolic gene clusters on pKW1. The mhpRBCDFETP cluster encodes enzymes for the dissimilation of 2,3-dihydroxyphenylpropionate in a novel arrangement characterized by the absence of a gene encoding 3-(3-hydroxyphenyl)propionate monooxygenase and the presence of a GntR-type regulator, whereas the nahINLOMKJ cluster encodes part of the naphthalene metabolic pathway. Transcription studies supported their possible involvement in chlorobenzene degradation. The upper pathway cluster, comprising genes encoding a chlorobenzene dioxygenase and a chlorobenzene dihydrodiol dehydrogenase, was localized on the chromosome. A high level of transcription in response to chlorobenzene revealed it to be crucial for chlorobenzene degradation. The chlorobenzene degradation pathway in strain GJ31 is thus a mosaic encoded by four gene clusters.en
dc.language.isoenen
dc.titleDegradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome.en
dc.typeArticleen
dc.contributor.departmentBergische Universität Wuppertal, Chemical Microbiology, D-42097 Wuppertal, Germany.en
dc.identifier.journalMicrobiology (Reading, England)en

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.