Strong interferon-inducing capacity of a highly virulent variant of influenza A virus strain PR8 with deletions in the NS1 gene.

2.50
Hdl Handle:
http://hdl.handle.net/10033/90955
Title:
Strong interferon-inducing capacity of a highly virulent variant of influenza A virus strain PR8 with deletions in the NS1 gene.
Authors:
Kochs, Georg; Martínez-Sobrido, Luis; Lienenklaus, Stefan; Weiss, Siegfried; García-Sastre, Adolfo; Staeheli, Peter
Abstract:
Influenza viruses lacking the interferon (IFN)-antagonistic non-structural NS1 protein are strongly attenuated. Here, we show that mutants of a highly virulent variant of A/PR/8/34 (H1N1) carrying either a complete deletion or C-terminal truncations of NS1 were far more potent inducers of IFN in infected mice than NS1 mutants derived from standard A/PR/8/34. Efficient induction of IFN correlated with successful initial virus replication in mouse lungs, indicating that the IFN response is boosted by enhanced viral activity. As the new NS1 mutants can be handled in standard biosafety laboratories, they represent convenient novel tools for studying virus-induced IFN expression in vivo.
Affiliation:
Department of Virology, University of Freiburg, D-79008 Freiburg, Germany. georg.kochs@uniklinik-freiburg.de
Citation:
Strong interferon-inducing capacity of a highly virulent variant of influenza A virus strain PR8 with deletions in the NS1 gene. 2009, 90 (Pt 12):2990-4 J. Gen. Virol.
Journal:
The Journal of general virology
Issue Date:
Dec-2009
URI:
http://hdl.handle.net/10033/90955
DOI:
10.1099/vir.0.015727-0
PubMed ID:
19726611
Type:
Article
Language:
en
ISSN:
1465-2099
Appears in Collections:
publications of the research group molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorKochs, Georgen
dc.contributor.authorMartínez-Sobrido, Luisen
dc.contributor.authorLienenklaus, Stefanen
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authorGarcía-Sastre, Adolfoen
dc.contributor.authorStaeheli, Peteren
dc.date.accessioned2010-02-01T15:32:02Z-
dc.date.available2010-02-01T15:32:02Z-
dc.date.issued2009-12-
dc.identifier.citationStrong interferon-inducing capacity of a highly virulent variant of influenza A virus strain PR8 with deletions in the NS1 gene. 2009, 90 (Pt 12):2990-4 J. Gen. Virol.en
dc.identifier.issn1465-2099-
dc.identifier.pmid19726611-
dc.identifier.doi10.1099/vir.0.015727-0-
dc.identifier.urihttp://hdl.handle.net/10033/90955-
dc.description.abstractInfluenza viruses lacking the interferon (IFN)-antagonistic non-structural NS1 protein are strongly attenuated. Here, we show that mutants of a highly virulent variant of A/PR/8/34 (H1N1) carrying either a complete deletion or C-terminal truncations of NS1 were far more potent inducers of IFN in infected mice than NS1 mutants derived from standard A/PR/8/34. Efficient induction of IFN correlated with successful initial virus replication in mouse lungs, indicating that the IFN response is boosted by enhanced viral activity. As the new NS1 mutants can be handled in standard biosafety laboratories, they represent convenient novel tools for studying virus-induced IFN expression in vivo.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshGene Deletionen
dc.subject.meshHumansen
dc.subject.meshInfluenza A Virus, H1N1 Subtypeen
dc.subject.meshInterferon-betaen
dc.subject.meshLungen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshMutationen
dc.subject.meshOrthomyxoviridae Infectionsen
dc.subject.meshViral Nonstructural Proteinsen
dc.subject.meshVirulenceen
dc.subject.meshVirus Replicationen
dc.titleStrong interferon-inducing capacity of a highly virulent variant of influenza A virus strain PR8 with deletions in the NS1 gene.en
dc.typeArticleen
dc.contributor.departmentDepartment of Virology, University of Freiburg, D-79008 Freiburg, Germany. georg.kochs@uniklinik-freiburg.deen
dc.identifier.journalThe Journal of general virologyen

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