2.50
Hdl Handle:
http://hdl.handle.net/10033/94153
Title:
Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity.
Authors:
Ukena, Sya N; Singh, Anurag; Dringenberg, Ulrike; Engelhardt, Regina; Seidler, Ursula; Hansen, Wiebke; Bleich, André; Bruder, Dunja; Franzke, Anke; Rogler, Gerhard; Suerbaum, Sebastian; Buer, Jan; Gunzer, Florian; Westendorf, Astrid M
Abstract:
BACKGROUND: Probiotics are proposed to positively modulate the intestinal epithelial barrier formed by intestinal epithelial cells (IECs) and intercellular junctions. Disruption of this border alters paracellular permeability and is a key mechanism for the development of enteric infections and inflammatory bowel diseases (IBDs). METHODOLOGY AND PRINCIPAL FINDINGS: To study the in vivo effect of probiotic Escherichia coli Nissle 1917 (EcN) on the stabilization of the intestinal barrier under healthy conditions, germfree mice were colonized with EcN or K12 E. coli strain MG1655. IECs were isolated and analyzed for gene and protein expression of the tight junction molecules ZO-1 and ZO-2. Then, in order to analyze beneficial effects of EcN under inflammatory conditions, the probiotic was orally administered to BALB/c mice with acute dextran sodium sulfate (DSS) induced colitis. Colonization of gnotobiotic mice with EcN resulted in an up-regulation of ZO-1 in IECs at both mRNA and protein levels. EcN administration to DSS-treated mice reduced the loss of body weight and colon shortening. In addition, infiltration of the colon with leukocytes was ameliorated in EcN inoculated mice. Acute DSS colitis did not result in an anion secretory defect, but abrogated the sodium absorptive function of the mucosa. Additionally, intestinal barrier function was severely affected as evidenced by a strong increase in the mucosal uptake of Evans blue in vivo. Concomitant administration of EcN to DSS treated animals resulted in a significant protection against intestinal barrier dysfunction and IECs isolated from these mice exhibited a more pronounced expression of ZO-1. CONCLUSION AND SIGNIFICANCE: This study convincingly demonstrates that probiotic EcN is able to mediate up-regulation of ZO-1 expression in murine IECs and confer protection from the DSS colitis-associated increase in mucosal permeability to luminal substances.
Affiliation:
Department of Mucosal Immunity, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity. 2007, 2 (12):e1308 PLoS ONE
Journal:
PloS one
Issue Date:
2007
URI:
http://hdl.handle.net/10033/94153
DOI:
10.1371/journal.pone.0001308
PubMed ID:
18074031
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of RG Mucosal Immunity (MI)

Full metadata record

DC FieldValue Language
dc.contributor.authorUkena, Sya Nen
dc.contributor.authorSingh, Anuragen
dc.contributor.authorDringenberg, Ulrikeen
dc.contributor.authorEngelhardt, Reginaen
dc.contributor.authorSeidler, Ursulaen
dc.contributor.authorHansen, Wiebkeen
dc.contributor.authorBleich, Andréen
dc.contributor.authorBruder, Dunjaen
dc.contributor.authorFranzke, Ankeen
dc.contributor.authorRogler, Gerharden
dc.contributor.authorSuerbaum, Sebastianen
dc.contributor.authorBuer, Janen
dc.contributor.authorGunzer, Florianen
dc.contributor.authorWestendorf, Astrid Men
dc.date.accessioned2010-03-12T09:20:52Z-
dc.date.available2010-03-12T09:20:52Z-
dc.date.issued2007-
dc.identifier.citationProbiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity. 2007, 2 (12):e1308 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid18074031-
dc.identifier.doi10.1371/journal.pone.0001308-
dc.identifier.urihttp://hdl.handle.net/10033/94153-
dc.description.abstractBACKGROUND: Probiotics are proposed to positively modulate the intestinal epithelial barrier formed by intestinal epithelial cells (IECs) and intercellular junctions. Disruption of this border alters paracellular permeability and is a key mechanism for the development of enteric infections and inflammatory bowel diseases (IBDs). METHODOLOGY AND PRINCIPAL FINDINGS: To study the in vivo effect of probiotic Escherichia coli Nissle 1917 (EcN) on the stabilization of the intestinal barrier under healthy conditions, germfree mice were colonized with EcN or K12 E. coli strain MG1655. IECs were isolated and analyzed for gene and protein expression of the tight junction molecules ZO-1 and ZO-2. Then, in order to analyze beneficial effects of EcN under inflammatory conditions, the probiotic was orally administered to BALB/c mice with acute dextran sodium sulfate (DSS) induced colitis. Colonization of gnotobiotic mice with EcN resulted in an up-regulation of ZO-1 in IECs at both mRNA and protein levels. EcN administration to DSS-treated mice reduced the loss of body weight and colon shortening. In addition, infiltration of the colon with leukocytes was ameliorated in EcN inoculated mice. Acute DSS colitis did not result in an anion secretory defect, but abrogated the sodium absorptive function of the mucosa. Additionally, intestinal barrier function was severely affected as evidenced by a strong increase in the mucosal uptake of Evans blue in vivo. Concomitant administration of EcN to DSS treated animals resulted in a significant protection against intestinal barrier dysfunction and IECs isolated from these mice exhibited a more pronounced expression of ZO-1. CONCLUSION AND SIGNIFICANCE: This study convincingly demonstrates that probiotic EcN is able to mediate up-regulation of ZO-1 expression in murine IECs and confer protection from the DSS colitis-associated increase in mucosal permeability to luminal substances.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBase Sequenceen
dc.subject.meshBlotting, Westernen
dc.subject.meshColitisen
dc.subject.meshDNA Primersen
dc.subject.meshEscherichia colien
dc.subject.meshFemaleen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshGerm-Free Lifeen
dc.subject.meshIntestinal Mucosaen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshProbioticsen
dc.titleProbiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Mucosal Immunity, Helmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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