2024-03-28T22:56:29Zhttp://repository.helmholtz-hzi.de/oai/requestoai:repository.helmholtz-hzi.de:10033/86182019-08-30T11:32:39Zcom_10033_6814col_10033_6885
1995-09
urn:hdl:10033/8618
Interaction of Listeria monocytogenes with mouse dendritic cells.
Guzman, C A
Rohde, M
Chakraborty, T
Domann, E
Hudel, M
Wehland, J
Timmis, K N
2007-02-20T13:12:44Z
1995-09
2007-02-20T13:12:44Z
1995-09
Infection and Immunity 1995 63(9):3665-3673
0019-9567
1098-5522
7642305
http://hdl.handle.net/10033/8618
173508
en_US
oai:repository.helmholtz-hzi.de:10033/86352019-08-30T11:32:40Zcom_10033_6814col_10033_6885
1995-12
urn:hdl:10033/8635
Localization of the stress protein SP21 in indole-induced spores, fruiting bodies, and heat-shocked cells of Stigmatella aurantiaca.
Lünsdorf, H
Schairer, H U
Heidelbach, M
2007-02-20T13:31:37Z
1995-12
2007-02-20T13:31:37Z
1995-12
Journal of Bacteriology 1995 177(24):7092-7099
0021-9193
1098-5530
8522514
http://hdl.handle.net/10033/8635
177586
en_US
oai:repository.helmholtz-hzi.de:10033/86382019-08-30T11:32:16Zcom_10033_6814col_10033_6885
1997-04
urn:hdl:10033/8638
Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains.
Klee, S R
Tzschaschel, B D
Timmis, K N
Guzman, C A
2007-02-20T13:33:51Z
1997-04
2007-02-20T13:33:51Z
1997-04
Journal of Bacteriology 1997 179(7):2421-2425
0021-9193
1098-5530
9079931
http://hdl.handle.net/10033/8638
178982
en_US
oai:repository.helmholtz-hzi.de:10033/86392019-08-30T11:32:40Zcom_10033_6814col_10033_6885
2003-09
urn:hdl:10033/8639
Intracellular Survival of Streptococcus pyogenes in Polymorphonuclear Cells Results in Increased Bacterial Virulence
Medina, Eva
Rohde, Manfred
Chhatwal, Gursharan S.
2007-02-20T13:34:24Z
2003-09
2007-02-20T13:34:24Z
2003-09
Infection and Immunity 2003 71(9):5376-5380
0019-9567
1098-5522
12933887
10.1128/IAI.71.9.5376-5380.2003
http://hdl.handle.net/10033/8639
187312
en_US
Copyright © 2003, American Society for Microbiology
American Society for Microbiology
oai:repository.helmholtz-hzi.de:10033/86032019-08-30T11:24:31Zcom_10033_6814col_10033_6885
1992-08
urn:hdl:10033/8603
Construction of stable LamB-Shiga toxin B subunit hybrids: analysis of expression in Salmonella typhimurium aroA strains and stimulation of B subunit-specific mucosal and serum antibody responses.
Su, G F
Brahmbhatt, H N
Wehland, J
Rohde, Manfred
Timmis, K N
Images
2007-02-20T12:56:07Z
1992-08
2007-02-20T12:56:07Z
1992-08
Infection and Immunity 1992 60(8):3345-3359
0019-9567
1098-5522
1639503
http://hdl.handle.net/10033/8603
257321
en_US
oai:repository.helmholtz-hzi.de:10033/86062019-08-30T11:24:31Zcom_10033_6814col_10033_6885
1991-05
urn:hdl:10033/8606
Production of recombinant Bordetella pertussis serotype 2 fimbriae in Bordetella parapertussis and Bordetella bronchiseptica: utility of Escherichia coli gene expression signals.
Walker, M J
Guzmán, C A
Rohde, Manfred
Timmis, K N
Images
2007-02-20T12:59:02Z
1991-05
2007-02-20T12:59:02Z
1991-05
Infection and Immunity 1991 59(5):1739-1746
0019-9567
1098-5522
1708358
http://hdl.handle.net/10033/8606
257910
en_US
oai:repository.helmholtz-hzi.de:10033/86082019-08-30T11:24:31Zcom_10033_6814col_10033_6885
1991-10
urn:hdl:10033/8608
Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.
Kadurugamuwa, J L
Rohde, Manfred
Wehland, J
Timmis, K N
Images
2007-02-20T13:01:01Z
1991-10
2007-02-20T13:01:01Z
1991-10
Infection and Immunity 1991 59(10):3463-3471
0019-9567
1098-5522
1910001
http://hdl.handle.net/10033/8608
258907
en_US
oai:repository.helmholtz-hzi.de:10033/86092019-08-30T11:25:43Zcom_10033_6814col_10033_6885
1991-10
urn:hdl:10033/8609
Direct expression of Bordetella pertussis filamentous hemagglutinin in Escherichia coli and Salmonella typhimurium aroA.
Guzmán, C A
Walker, M J
Rohde, Manfred
Timmis, K N
Images
2007-02-20T13:01:36Z
1991-10
2007-02-20T13:01:36Z
1991-10
Infection and Immunity 1991 59(10):3787-3795
0019-9567
1098-5522
1716615
http://hdl.handle.net/10033/8609
258952
en_US
oai:repository.helmholtz-hzi.de:10033/86192019-08-30T11:25:11Zcom_10033_6814col_10033_6885
1991-11
urn:hdl:10033/8619
Construction of minitransposons for constitutive and inducible expression of pertussis toxin in bvg-negative Bordetella bronchiseptica.
Walker, M J
Rohde, Manfred
Wehland, J
Timmis, K N
Images
2007-02-20T13:15:13Z
1991-11
2007-02-20T13:15:13Z
1991-11
Infection and Immunity 1991 59(11):4238-4248
0019-9567
1098-5522
1682257
http://hdl.handle.net/10033/8619
259022
en_US
oai:repository.helmholtz-hzi.de:10033/86202019-08-30T11:25:43Zcom_10033_6814col_10033_6885
1991-11
urn:hdl:10033/8620
Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells.
Walker, M J
Wehland, J
Timmis, K N
Raupach, B
Schmidt, M A
Images
2007-02-20T13:15:51Z
1991-11
2007-02-20T13:15:51Z
1991-11
Infection and Immunity 1991 59(11):4249-4251
0019-9567
1098-5522
1718872
http://hdl.handle.net/10033/8620
259023
en_US
oai:repository.helmholtz-hzi.de:10033/86212019-08-30T11:26:12Zcom_10033_6814col_10033_6885
1994-09
urn:hdl:10033/8621
Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and -independent activation of the TOL meta operon requires the same cis-acting sequences within the Pm promoter.
Kessler, B
Marqués, S
Köhler, T
Ramos, J L
Timmis, K N
de Lorenzo, V
Images
2007-02-20T13:16:52Z
1994-09
2007-02-20T13:16:52Z
1994-09
Journal of Bacteriology 1994 176(17):5578-5582
0021-9193
1098-5530
8071244
http://hdl.handle.net/10033/8621
196754
en_US
oai:repository.helmholtz-hzi.de:10033/86242019-08-30T11:25:43Zcom_10033_6814col_10033_6885
1993-07
urn:hdl:10033/8624
Localization of the ActA polypeptide of Listeria monocytogenes in infected tissue culture cell lines: ActA is not associated with actin "comets".
Niebuhr, K
Chakraborty, T
Rohde, Manfred
Gazlig, T
Jansen, B
Köllner, P
Wehland, J
Images
2007-02-20T13:23:07Z
1993-07
2007-02-20T13:23:07Z
1993-07
Infection and Immunity 1993 61(7):2793-2802
0019-9567
1098-5522
8514381
http://hdl.handle.net/10033/8624
280923
en_US
oai:repository.helmholtz-hzi.de:10033/86252019-08-30T11:24:25Zcom_10033_6814col_10033_6885
1980-04
urn:hdl:10033/8625
Occurrence of Cytophagas in Sewage Plants
Güde, Hans
2007-02-20T13:23:51Z
1980-04
2007-02-20T13:23:51Z
1980-04
Applied and Environmental Microbiology 1980 39(4):756-763
0099-2240
1098-5336
http://hdl.handle.net/10033/8625
291415
en_US
oai:repository.helmholtz-hzi.de:10033/86322019-08-30T11:26:12Zcom_10033_6814col_10033_6885
1994-12
urn:hdl:10033/8632
Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.
Kloos, D U
Strätz, M
Güttler, A
Steffan, R J
Timmis, K N
Images
2007-02-20T13:29:29Z
1994-12
2007-02-20T13:29:29Z
1994-12
Journal of Bacteriology 1994 176(23):7352-7361
0021-9193
1098-5530
7961508
http://hdl.handle.net/10033/8632
197125
en_US
oai:repository.helmholtz-hzi.de:10033/86412019-08-30T11:25:43Zcom_10033_6814col_10033_6885
1991-02
urn:hdl:10033/8641
Immunocytochemical localization of the coenzyme F420-reducing hydrogenase in Methanosarcina barkeri Fusaro.
Lünsdorf, H
Niedrig, M
Fiebig, K
Images
2007-02-20T13:35:37Z
1991-02
2007-02-20T13:35:37Z
1991-02
Journal of Bacteriology 1991 173(3):978-984
0021-9193
1098-5530
1991734
http://hdl.handle.net/10033/8641
207214
en_US
oai:repository.helmholtz-hzi.de:10033/86442019-08-30T11:27:08Zcom_10033_6814col_10033_6885
1990-09
urn:hdl:10033/8644
Bacterial infection of wounds: fibronectin-mediated adherence group A and C streptococci to fibrin thrombi in vitro.
Chhatwal, G S
Valentin-Weigand, P
Timmis, K N
2007-02-20T13:37:09Z
1990-09
2007-02-20T13:37:09Z
1990-09
Infection and Immunity 1990 58(9):3015-3019
0019-9567
1098-5522
2201643
http://hdl.handle.net/10033/8644
313604
en_US
oai:repository.helmholtz-hzi.de:10033/86502019-08-30T11:25:38Zcom_10033_6814col_10033_6885
2003-12
urn:hdl:10033/8650
Identification of B- and T-Cell Epitopes within the Fibronectin-Binding Domain of the SfbI Protein of Streptococcus pyogenes
Schulze, Kai
Medina, Eva
Chhatwal, Gursharan S.
Guzmán, Carlos A.
2007-02-20T13:40:07Z
2003-12
2007-02-20T13:40:07Z
2003-12
Infection and Immunity 2003 71(12):7197-7201
0019-9567
1098-5522
14638816
10.1128/IAI.71.12.7197-7201.2003
http://hdl.handle.net/10033/8650
308920
en_US
Copyright © 2003, American Society for Microbiology
American Society for Microbiology
oai:repository.helmholtz-hzi.de:10033/86622019-08-30T11:26:13Zcom_10033_6814col_10033_6885
1985-12
urn:hdl:10033/8662
Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies
Tiedge, Henri
Lünsdorf, Heinrich
Schäfer, Günter
Schairer, Hans Ulrich
Images
2007-02-20T14:34:49Z
1985-12
2007-02-20T14:34:49Z
1985-12
Proceedings of the National Academy of Sciences of the United States of America 1985 82(23):7874-7878
0027-8424
1091-6490
http://hdl.handle.net/10033/8662
390872
en_US
oai:repository.helmholtz-hzi.de:10033/86652019-08-30T11:27:08Zcom_10033_6814col_10033_6885
2004-05
urn:hdl:10033/8665
Growth of Polychlorinated-Biphenyl-Degrading Bacteria in the Presence of Biphenyl and Chlorobiphenyls Generates Oxidative Stress and Massive Accumulation of Inorganic Polyphosphate
Chávez, Francisco P.
Lünsdorf, Heinrich
Jerez, Carlos A.
2007-02-20T14:36:38Z
2004-05
2007-02-20T14:36:38Z
2004-05
Applied and Environmental Microbiology 2004 70(5):3064-3072
0099-2240
1098-5336
15128568
10.1128/AEM.70.5.3064-3072.2004
http://hdl.handle.net/10033/8665
404396
en_US
Copyright © 2004, American Society for Microbiology
American Society for Microbiology
oai:repository.helmholtz-hzi.de:10033/87632019-08-30T11:26:13Zcom_10033_6814col_10033_6885
2006-06-27
urn:hdl:10033/8763
Cationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopy
Lünsdorf, Heinrich
Kristen, Ingeborg
Barth, Elke
Background Synthesis of cationic hydrous thorium dioxide colloids (ca. 1.0 to 1.7 nm) has been originally described by Müller [22] and Groot [11] and these have been used by Groot to stain acidic glucosaminoglycans for ultrastructure research of different tissues by conventional transmission electron microscopy. Results Synthesis of colloidal thorium dioxide has been modified and its use as a suitable stain of acidic mucopolysaccharides and other anionic biopolymers from bacteria, either as whole mount preparations or as preembedment labels, is described. The differences in stain behavior relative to commonly used rutheniumred-lysine and Alcian Blue™ electron dense acidic stains has been investigated and its use is exemplified for Pseudomonas aeruginosa adjacent cell wall biopolymers. For the first time thorificated biopolymers, i.e. bacterial outer cell wall layers, have been analysed at the ultrastructural level with electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI), leading to excellent contrast and signal strength for these extracellular biopolymers. Conclusion Application of cationic hydrous ThO2 colloids for tracing acidic groups of the bacterial surface and/or EPS has been shown to be rather effective by transmission electron microscopy. Because of its high electron density and its good diffusibility it stains and outlines electro-negative charges within these biopolymers. In combination with ESI, based on integrated energy-filtered electron microscopy (EFTEM) Th-densities and thus negative charge densities can be discriminated from other elemental densities, especially in environmental samples, such as biofilms.
2007-02-22T14:59:18Z
2006-06-27
2007-02-22T14:59:18Z
2006-06-27
BMC Microbiology 2006 6:59
1471-2180
16803626
10.1186/1471-2180-6-59
http://hdl.handle.net/10033/8763
1524781
en_US
http://www.biomedcentral.com/1471-2180/6/59
http://creativecommons.org/licenses/by/2.0
Copyright © 2006 Lünsdorf et al; licensee BioMed Central Ltd.
BioMed Central
oai:repository.helmholtz-hzi.de:10033/123072019-08-30T11:31:49Zcom_10033_6814col_10033_6885
2007-06-08T14:05:25Z
urn:hdl:10033/12307
7-O-malonyl macrolactin A, a new macrolactin antibiotic from Bacillus subtilis active against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of Burkholderia cepacia.
Romero-Tabarez, Magally
Jansen, Rolf
Sylla, Marita
Lünsdorf, Heinrich
Häussler, Susanne
Santosa, Dwi A
Timmis, Kenneth N
Molinari, Gabriella
We report here the discovery, isolation, and chemical and preliminary biological characterization of a new antibiotic compound, 7-O-malonyl macrolactin A (MMA), produced by a Bacillus subtilis soil isolate. MMA is a bacteriostatic antibiotic that inhibits a number of multidrug-resistant gram-positive bacterial pathogens, including methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of Burkholderia cepacia. MMA-treated staphylococci and enterococci were pseudomulticellular and exhibited multiple asymmetric initiation points of septum formation, indicating that MMA may inhibit a cell division function.
2007-06-08T14:05:25Z
2007-06-08T14:05:25Z
2006-05-01
Article
Antimicrob. Agents Chemother. 2006, 50(5):1701-9
0066-4804
16641438
10.1128/AAC.50.5.1701-1709.2006
http://hdl.handle.net/10033/12307
en
oai:repository.helmholtz-hzi.de:10033/146302019-08-30T11:35:13Zcom_10033_6814col_10033_6885
2007-11-19T14:44:40Z
urn:hdl:10033/14630
Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.
Seibel, Jürgen
Hellmuth, Hendrik
Hofer, Bernd
Kicinska, Anna-Maria
Schmalbruch, Bodo
Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.
2007-11-19T14:44:40Z
2007-11-19T14:44:40Z
2006-02-01
Article
Chembiochem 2006, 7(2):310-20
1439-4227
16416490
10.1002/cbic.200500350
http://hdl.handle.net/10033/14630
en
oai:repository.helmholtz-hzi.de:10033/6208112019-08-30T11:29:17Zcom_10033_6814col_10033_6885
2017-02-07T14:45:49Z
urn:hdl:10033/620811
Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400.
Seeger, M
Timmis, K N
Hofer, B
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Metabolism of 21 chlorobiphenyls by the enzymes of the upper biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. strain LB400 was investigated by using recombinant strains harboring gene cassettes containing bphABC or bphABCD. The enzymes of the upper pathway were generally able to metabolize mono- and dichlorinated biphenyls but only partially transform most trichlorinated congeners investigated: 14 of 15 mono- and dichlorinated and 2 of 6 trichlorinated congeners were converted into benzoates. All mono- and at least 8 of 12 dichlorinated congeners were attacked by the bphA-encoded biphenyl dioxygenase virtually exclusively at ortho and meta carbons. This enzyme exhibited a high degree of selectivity for the aromatic ring to be attacked, with the order of ring preference being non- > ortho- > meta- > para-substituted for mono- and dichlorinated congeners. The influence of the chlorine substitution pattern of the metabolized ring on benzoate formation resembled its influence on the reactivity of initial dioxygenation, suggesting that the rate of benzoate formation may frequently be determined by the rate of initial attack. The absorption spectra of phenylhexadienoates formed correlated with the presence or absence of a chlorine substituent at an ortho position.
2017-02-07T14:45:49Z
2017-02-07T14:45:49Z
1995-07
Article
Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. 1995, 61 (7):2654-8 Appl. Environ. Microbiol.
0099-2240
7618878
http://hdl.handle.net/10033/620811
Applied and environmental microbiology
en
http://creativecommons.org/licenses/by-nc-sa/4.0/