2024-03-29T10:58:48Zhttp://repository.helmholtz-hzi.de/oai/requestoai:repository.helmholtz-hzi.de:10033/86182019-08-30T11:32:39Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Guzman, C A
author
Rohde, M
author
Chakraborty, T
author
Domann, E
author
Hudel, M
author
Wehland, J
author
Timmis, K N
author
1995-09
Infection and Immunity 1995 63(9):3665-3673
0019-9567
1098-5522
7642305
http://hdl.handle.net/10033/8618
173508
Interaction of Listeria monocytogenes with mouse dendritic cells.
oai:repository.helmholtz-hzi.de:10033/86352019-08-30T11:32:40Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Lünsdorf, H
author
Schairer, H U
author
Heidelbach, M
author
1995-12
Journal of Bacteriology 1995 177(24):7092-7099
0021-9193
1098-5530
8522514
http://hdl.handle.net/10033/8635
177586
Localization of the stress protein SP21 in indole-induced spores, fruiting bodies, and heat-shocked cells of Stigmatella aurantiaca.
oai:repository.helmholtz-hzi.de:10033/86382019-08-30T11:32:16Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Klee, S R
author
Tzschaschel, B D
author
Timmis, K N
author
Guzman, C A
author
1997-04
Journal of Bacteriology 1997 179(7):2421-2425
0021-9193
1098-5530
9079931
http://hdl.handle.net/10033/8638
178982
Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains.
oai:repository.helmholtz-hzi.de:10033/86392019-08-30T11:32:40Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Medina, Eva
author
Rohde, Manfred
author
Chhatwal, Gursharan S.
author
2003-09
Infection and Immunity 2003 71(9):5376-5380
0019-9567
1098-5522
12933887
10.1128/IAI.71.9.5376-5380.2003
http://hdl.handle.net/10033/8639
187312
Intracellular Survival of Streptococcus pyogenes in Polymorphonuclear Cells Results in Increased Bacterial Virulence
oai:repository.helmholtz-hzi.de:10033/86032019-08-30T11:24:31Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Su, G F
author
Brahmbhatt, H N
author
Wehland, J
author
Rohde, Manfred
author
Timmis, K N
author
1992-08
Images
Infection and Immunity 1992 60(8):3345-3359
0019-9567
1098-5522
1639503
http://hdl.handle.net/10033/8603
257321
Construction of stable LamB-Shiga toxin B subunit hybrids: analysis of expression in Salmonella typhimurium aroA strains and stimulation of B subunit-specific mucosal and serum antibody responses.
oai:repository.helmholtz-hzi.de:10033/86062019-08-30T11:24:31Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Walker, M J
author
Guzmán, C A
author
Rohde, Manfred
author
Timmis, K N
author
1991-05
Images
Infection and Immunity 1991 59(5):1739-1746
0019-9567
1098-5522
1708358
http://hdl.handle.net/10033/8606
257910
Production of recombinant Bordetella pertussis serotype 2 fimbriae in Bordetella parapertussis and Bordetella bronchiseptica: utility of Escherichia coli gene expression signals.
oai:repository.helmholtz-hzi.de:10033/86082019-08-30T11:24:31Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Kadurugamuwa, J L
author
Rohde, Manfred
author
Wehland, J
author
Timmis, K N
author
1991-10
Images
Infection and Immunity 1991 59(10):3463-3471
0019-9567
1098-5522
1910001
http://hdl.handle.net/10033/8608
258907
Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.
oai:repository.helmholtz-hzi.de:10033/86092019-08-30T11:25:43Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Guzmán, C A
author
Walker, M J
author
Rohde, Manfred
author
Timmis, K N
author
1991-10
Images
Infection and Immunity 1991 59(10):3787-3795
0019-9567
1098-5522
1716615
http://hdl.handle.net/10033/8609
258952
Direct expression of Bordetella pertussis filamentous hemagglutinin in Escherichia coli and Salmonella typhimurium aroA.
oai:repository.helmholtz-hzi.de:10033/86192019-08-30T11:25:11Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Walker, M J
author
Rohde, Manfred
author
Wehland, J
author
Timmis, K N
author
1991-11
Images
Infection and Immunity 1991 59(11):4238-4248
0019-9567
1098-5522
1682257
http://hdl.handle.net/10033/8619
259022
Construction of minitransposons for constitutive and inducible expression of pertussis toxin in bvg-negative Bordetella bronchiseptica.
oai:repository.helmholtz-hzi.de:10033/86202019-08-30T11:25:43Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Walker, M J
author
Wehland, J
author
Timmis, K N
author
Raupach, B
author
Schmidt, M A
author
1991-11
Images
Infection and Immunity 1991 59(11):4249-4251
0019-9567
1098-5522
1718872
http://hdl.handle.net/10033/8620
259023
Characterization of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells.
oai:repository.helmholtz-hzi.de:10033/86212019-08-30T11:26:12Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Kessler, B
author
Marqués, S
author
Köhler, T
author
Ramos, J L
author
Timmis, K N
author
de Lorenzo, V
author
1994-09
Images
Journal of Bacteriology 1994 176(17):5578-5582
0021-9193
1098-5530
8071244
http://hdl.handle.net/10033/8621
196754
Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and -independent activation of the TOL meta operon requires the same cis-acting sequences within the Pm promoter.
oai:repository.helmholtz-hzi.de:10033/86242019-08-30T11:25:43Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Niebuhr, K
author
Chakraborty, T
author
Rohde, Manfred
author
Gazlig, T
author
Jansen, B
author
Köllner, P
author
Wehland, J
author
1993-07
Images
Infection and Immunity 1993 61(7):2793-2802
0019-9567
1098-5522
8514381
http://hdl.handle.net/10033/8624
280923
Localization of the ActA polypeptide of Listeria monocytogenes in infected tissue culture cell lines: ActA is not associated with actin "comets".
oai:repository.helmholtz-hzi.de:10033/86252019-08-30T11:24:25Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Güde, Hans
author
1980-04
Applied and Environmental Microbiology 1980 39(4):756-763
0099-2240
1098-5336
http://hdl.handle.net/10033/8625
291415
Occurrence of Cytophagas in Sewage Plants
oai:repository.helmholtz-hzi.de:10033/86322019-08-30T11:26:12Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Kloos, D U
author
Strätz, M
author
Güttler, A
author
Steffan, R J
author
Timmis, K N
author
1994-12
Images
Journal of Bacteriology 1994 176(23):7352-7361
0021-9193
1098-5530
7961508
http://hdl.handle.net/10033/8632
197125
Inducible cell lysis system for the study of natural transformation and environmental fate of DNA released by cell death.
oai:repository.helmholtz-hzi.de:10033/86412019-08-30T11:25:43Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Lünsdorf, H
author
Niedrig, M
author
Fiebig, K
author
1991-02
Images
Journal of Bacteriology 1991 173(3):978-984
0021-9193
1098-5530
1991734
http://hdl.handle.net/10033/8641
207214
Immunocytochemical localization of the coenzyme F420-reducing hydrogenase in Methanosarcina barkeri Fusaro.
oai:repository.helmholtz-hzi.de:10033/86442019-08-30T11:27:08Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Chhatwal, G S
author
Valentin-Weigand, P
author
Timmis, K N
author
1990-09
Infection and Immunity 1990 58(9):3015-3019
0019-9567
1098-5522
2201643
http://hdl.handle.net/10033/8644
313604
Bacterial infection of wounds: fibronectin-mediated adherence group A and C streptococci to fibrin thrombi in vitro.
oai:repository.helmholtz-hzi.de:10033/86502019-08-30T11:25:38Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Schulze, Kai
author
Medina, Eva
author
Chhatwal, Gursharan S.
author
Guzmán, Carlos A.
author
2003-12
Infection and Immunity 2003 71(12):7197-7201
0019-9567
1098-5522
14638816
10.1128/IAI.71.12.7197-7201.2003
http://hdl.handle.net/10033/8650
308920
Identification of B- and T-Cell Epitopes within the Fibronectin-Binding Domain of the SfbI Protein of Streptococcus pyogenes
oai:repository.helmholtz-hzi.de:10033/86622019-08-30T11:26:13Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Tiedge, Henri
author
Lünsdorf, Heinrich
author
Schäfer, Günter
author
Schairer, Hans Ulrich
author
1985-12
Images
Proceedings of the National Academy of Sciences of the United States of America 1985 82(23):7874-7878
0027-8424
1091-6490
http://hdl.handle.net/10033/8662
390872
Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies
oai:repository.helmholtz-hzi.de:10033/86652019-08-30T11:27:08Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Chávez, Francisco P.
author
Lünsdorf, Heinrich
author
Jerez, Carlos A.
author
2004-05
Applied and Environmental Microbiology 2004 70(5):3064-3072
0099-2240
1098-5336
15128568
10.1128/AEM.70.5.3064-3072.2004
http://hdl.handle.net/10033/8665
404396
Growth of Polychlorinated-Biphenyl-Degrading Bacteria in the Presence of Biphenyl and Chlorobiphenyls Generates Oxidative Stress and Massive Accumulation of Inorganic Polyphosphate
oai:repository.helmholtz-hzi.de:10033/87632019-08-30T11:26:13Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Lünsdorf, Heinrich
author
Kristen, Ingeborg
author
Barth, Elke
author
2006-06-27
Background Synthesis of cationic hydrous thorium dioxide colloids (ca. 1.0 to 1.7 nm) has been originally described by Müller [22] and Groot [11] and these have been used by Groot to stain acidic glucosaminoglycans for ultrastructure research of different tissues by conventional transmission electron microscopy. Results Synthesis of colloidal thorium dioxide has been modified and its use as a suitable stain of acidic mucopolysaccharides and other anionic biopolymers from bacteria, either as whole mount preparations or as preembedment labels, is described. The differences in stain behavior relative to commonly used rutheniumred-lysine and Alcian Blue™ electron dense acidic stains has been investigated and its use is exemplified for Pseudomonas aeruginosa adjacent cell wall biopolymers. For the first time thorificated biopolymers, i.e. bacterial outer cell wall layers, have been analysed at the ultrastructural level with electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI), leading to excellent contrast and signal strength for these extracellular biopolymers. Conclusion Application of cationic hydrous ThO2 colloids for tracing acidic groups of the bacterial surface and/or EPS has been shown to be rather effective by transmission electron microscopy. Because of its high electron density and its good diffusibility it stains and outlines electro-negative charges within these biopolymers. In combination with ESI, based on integrated energy-filtered electron microscopy (EFTEM) Th-densities and thus negative charge densities can be discriminated from other elemental densities, especially in environmental samples, such as biofilms.
BMC Microbiology 2006 6:59
1471-2180
16803626
10.1186/1471-2180-6-59
http://hdl.handle.net/10033/8763
1524781
Cationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopy
oai:repository.helmholtz-hzi.de:10033/123072019-08-30T11:31:49Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Romero-Tabarez, Magally
author
Jansen, Rolf
author
Sylla, Marita
author
Lünsdorf, Heinrich
author
Häussler, Susanne
author
Santosa, Dwi A
author
Timmis, Kenneth N
author
Molinari, Gabriella
author
2006-05-01
We report here the discovery, isolation, and chemical and preliminary biological characterization of a new antibiotic compound, 7-O-malonyl macrolactin A (MMA), produced by a Bacillus subtilis soil isolate. MMA is a bacteriostatic antibiotic that inhibits a number of multidrug-resistant gram-positive bacterial pathogens, including methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of Burkholderia cepacia. MMA-treated staphylococci and enterococci were pseudomulticellular and exhibited multiple asymmetric initiation points of septum formation, indicating that MMA may inhibit a cell division function.
Antimicrob. Agents Chemother. 2006, 50(5):1701-9
0066-4804
16641438
10.1128/AAC.50.5.1701-1709.2006
http://hdl.handle.net/10033/12307
7-O-malonyl macrolactin A, a new macrolactin antibiotic from Bacillus subtilis active against methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of Burkholderia cepacia.
oai:repository.helmholtz-hzi.de:10033/146302019-08-30T11:35:13Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Seibel, Jürgen
author
Hellmuth, Hendrik
author
Hofer, Bernd
author
Kicinska, Anna-Maria
author
Schmalbruch, Bodo
author
2006-02-01
Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.
Chembiochem 2006, 7(2):310-20
1439-4227
16416490
10.1002/cbic.200500350
http://hdl.handle.net/10033/14630
Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.
oai:repository.helmholtz-hzi.de:10033/6208112019-08-30T11:29:17Zcom_10033_6814col_10033_6885
00925njm 22002777a 4500
dc
Seeger, M
author
Timmis, K N
author
Hofer, B
author
1995-07
Metabolism of 21 chlorobiphenyls by the enzymes of the upper biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. strain LB400 was investigated by using recombinant strains harboring gene cassettes containing bphABC or bphABCD. The enzymes of the upper pathway were generally able to metabolize mono- and dichlorinated biphenyls but only partially transform most trichlorinated congeners investigated: 14 of 15 mono- and dichlorinated and 2 of 6 trichlorinated congeners were converted into benzoates. All mono- and at least 8 of 12 dichlorinated congeners were attacked by the bphA-encoded biphenyl dioxygenase virtually exclusively at ortho and meta carbons. This enzyme exhibited a high degree of selectivity for the aromatic ring to be attacked, with the order of ring preference being non- > ortho- > meta- > para-substituted for mono- and dichlorinated congeners. The influence of the chlorine substitution pattern of the metabolized ring on benzoate formation resembled its influence on the reactivity of initial dioxygenation, suggesting that the rate of benzoate formation may frequently be determined by the rate of initial attack. The absorption spectra of phenylhexadienoates formed correlated with the presence or absence of a chlorine substituent at an ortho position.
Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. 1995, 61 (7):2654-8 Appl. Environ. Microbiol.
0099-2240
7618878
http://hdl.handle.net/10033/620811
Applied and environmental microbiology
Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400.