2024-03-28T21:31:29Zhttp://repository.helmholtz-hzi.de/oai/requestoai:repository.helmholtz-hzi.de:10033/85032019-08-30T11:32:38Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
Evaluation of sequence motifs found in scaffold/matrix-attached regions (S/MARs)
Liebich, I.
Bode, J.
Reuter, I.
Wingender, E.
2002-08-01
2007-02-19T08:57:29Z
2002-08-01
2007-02-19T08:57:29Z
2002-08-01
2007-02-19T08:57:29Z
2002-08-01
Nucleic Acids Research 2002 30(15):3433-3442
0305-1048
1362-4962
12140328
http://hdl.handle.net/10033/8503
137072
en_US
http://www.nar.oupjournals.org/content/vol30/issue15/
Copyright © 2002 Oxford University Press
Oxford University Press
oai:repository.helmholtz-hzi.de:10033/86812019-08-30T11:25:40Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
Nuclear scaffold/matrix attached region modules linked to a transcription unit are sufficient for replication and maintenance of a mammalian episome
Jenke, Andreas C. W.
Stehle, Isa M.
Herrmann, Frank
Eisenberger, Tobias
Baiker, Armin
Bode, Jürgen
Fackelmayer, Frank O.
Lipps, Hans J.
2004-08-03
2007-02-21T08:17:26Z
2004-08-03
2007-02-21T08:17:26Z
2004-08-03
2007-02-21T08:17:26Z
2004-08-03
Proceedings of the National Academy of Sciences of the United States of America 2004 101(31):11322-11327
0027-8424
1091-6490
15272077
10.1073/pnas.0401355101
http://hdl.handle.net/10033/8681
509201
en_US
Copyright © 2004, The National Academy of Sciences
National Academy of Sciences
oai:repository.helmholtz-hzi.de:10033/86952019-08-30T11:24:27Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
Performance of Genomic Bordering Elements at Predefined Genomic Loci
Goetze, Sandra
Baer, Alexandra
Winkelmann, Silke
Nehlsen, Kristina
Seibler, Jost
Maass, Karin
Bode, Jürgen
2005-03
2007-02-21T08:35:35Z
2005-03
2007-02-21T08:35:35Z
2005-03
2007-02-21T08:35:35Z
2005-03
Molecular and Cellular Biology 2005 25(6):2260-2272
0270-7306
1098-5549
15743822
10.1128/MCB.25.6.2260-2272.2005
http://hdl.handle.net/10033/8695
1061597
en_US
Copyright © 2005, American Society for Microbiology
American Society for Microbiology
oai:repository.helmholtz-hzi.de:10033/87432019-08-30T11:30:29Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
Enhanceosome Formation over the Beta Interferon Promoter Underlies a Remote-Control Mechanism Mediated by YY1 and YY2
Klar, Martin
Bode, Juergen
2005-11
2007-02-22T13:38:14Z
2005-11
2007-02-22T13:38:14Z
2005-11
2007-02-22T13:38:14Z
2005-11
Molecular and Cellular Biology 2005 25(22):10159-10170
0270-7306
1098-5549
16260628
10.1128/MCB.25.22.10159-10170.2005
http://hdl.handle.net/10033/8743
1280260
en_US
Copyright © 2005, American Society for Microbiology
American Society for Microbiology
oai:repository.helmholtz-hzi.de:10033/360922019-08-30T11:26:05Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
T(3;7)(q27;q32) fuses BCL6 to a non-coding region at FRA7H near miR-29.
Schneider, B
Nagel, S
Kaufmann, M
Winkelmann, S
Bode, J
Drexler, H G
MacLeod, R A F
2008-08-21T10:35:07Z
2008-08-21T10:35:07Z
2008-08-21T10:35:07Z
2008-06
Article
T(3;7)(q27;q32) fuses BCL6 to a non-coding region at FRA7H near miR-29. 2008, 22 (6):1262-6 Leukemia
1476-5551
17989715
10.1038/sj.leu.2405025
http://hdl.handle.net/10033/36092
Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K
en
oai:repository.helmholtz-hzi.de:10033/766532019-08-30T11:34:22Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties.
Qiao, Junhua
Oumard, André
Wegloehner, Wolfgang
Bode, Juergen
Department of Molecular Biotechnology/Epigenetic Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Site-specific recombinases have revolutionized the systematic generation of transgenic cell lines and embryonic stem cells/animals and will ultimately also reveal their potential in the genetic modification of induced pluripotent stem cells. Introduced in 1994, our Flp recombinase-mediated cassette exchange strategy permits the exchange of a target cassette for a cassette with the gene of interest, introduced as a part of an exchange vector. The process is "clean" in the sense that it does not co-introduce prokaryotic vector parts; neither does it leave behind a selection marker. Stringent selection principles provide master cell lines permitting subsequent recombinase-mediated cassette exchange cycles in the absence of a drug selection and with a considerable efficiency (approximately 10%). Exemplified by Chinese hamster ovary cells, the strategy proves to be successful even for cell lines with an unstable genotype.
2009-08-07T09:05:59Z
2009-08-07T09:05:59Z
2009-08-07T09:05:59Z
2009-07-24
Article
Novel tag-and-exchange (RMCE) strategies generate master cell clones with predictable and stable transgene expression properties. 2009, 390 (4):579-94 J. Mol. Biol.
1089-8638
19447116
10.1016/j.jmb.2009.05.012
http://hdl.handle.net/10033/76653
Journal of molecular biology
en
oai:repository.helmholtz-hzi.de:10033/2461522019-08-30T11:28:24Zcom_10033_6824com_10033_6823com_10033_6820col_10033_6892
YY1-Binding Sites Provide Central Switch Functions in the PARP-1 Gene Expression Network.
Doetsch, Martina
Gluch, Angela
Poznanović, Goran
Bode, Juergen
Vidaković, Melita
Helmholtz Centre for Infection Research/Epigenetic Regulation, Braunschweig, Germany.
Evidence is presented for the involvement of the interplay between transcription factor Yin Yang 1 (YY1) and poly(ADP-ribose) polymerase-1 (PARP-1) in the regulation of mouse PARP-1 gene (muPARP-1) promoter activity. We identified potential YY1 binding motifs (BM) at seven positions in the muPARP-1 core-promoter (-574/+200). Binding of YY1 was observed by the electrophoretic supershift assay using anti-YY1 antibody and linearized or supercoiled forms of plasmids bearing the core promoter, as well as with 30 bp oligonucleotide probes containing the individual YY1 binding motifs and four muPARP-1 promoter fragments. We detected YY1 binding to BM1 (-587/-558), BM4 (-348/-319) and a very prominent association with BM7 (+86/+115). Inspection of BM7 reveals overlap of the muPARP-1 translation start site with the Kozak sequence and YY1 and PARP-1 recognition sites. Site-directed mutagenesis of the YY1 and PARP-1 core motifs eliminated protein binding and showed that YY1 mediates PARP-1 binding next to the Kozak sequence. Transfection experiments with a reporter gene under the control of the muPARP-1 promoter revealed that YY1 binding to BM1 and BM4 independently repressed the promoter. Mutations at these sites prevented YY1 binding, allowing for increased reporter gene activity. In PARP-1 knockout cells subjected to PARP-1 overexpression, effects similar to YY1 became apparent; over expression of YY1 and PARP-1 revealed their synergistic action. Together with our previous findings these results expand the PARP-1 autoregulatory loop principle by YY1 actions, implying rigid limitation of muPARP-1 expression. The joint actions of PARP-1 and YY1 emerge as important contributions to cell homeostasis.
2012-09-27T13:01:41Z
2012-09-27T13:01:41Z
2012-09-27T13:01:41Z
2012
Article
YY1-Binding Sites Provide Central Switch Functions in the PARP-1 Gene Expression Network. 2012, 7 (8):e44125 PLoS ONE
1932-6203
22937159
10.1371/journal.pone.0044125
http://hdl.handle.net/10033/246152
PloS one
en
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