publications of the research group Microbial Proteomics (MPRO)
http://hdl.handle.net/10033/620560
2024-03-29T12:07:20Z
2024-03-29T12:07:20Z
Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation
Heimes, A.
Brodhagen, J.
Weikard, R.
Becker, D.
Meyerholz, M. M.
Petzl, W.
Zerbe, H.
Schuberth, H. J.
Hoedemaker, M.
Schmicke, M.
Engelmann, S.
Kühn, C.
http://hdl.handle.net/10033/623236
2022-06-21T01:54:25Z
2020-07-01T00:00:00Z
Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation
Heimes, A.; Brodhagen, J.; Weikard, R.; Becker, D.; Meyerholz, M. M.; Petzl, W.; Zerbe, H.; Schuberth, H. J.; Hoedemaker, M.; Schmicke, M.; Engelmann, S.; Kühn, C.
Infection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Association
Infection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Association
2020-07-01T00:00:00Z
Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation.
Heimes, A
Brodhagen, J
Weikard, R
Becker, D
Meyerholz, M M
Petzl, W
Zerbe, H
Schuberth, H-J
Hoedemaker, M
Schmicke, M
Engelmann, S
Kühn, C
http://hdl.handle.net/10033/623221
2022-06-15T02:52:43Z
2020-04-16T00:00:00Z
Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation.
Heimes, A; Brodhagen, J; Weikard, R; Becker, D; Meyerholz, M M; Petzl, W; Zerbe, H; Schuberth, H-J; Hoedemaker, M; Schmicke, M; Engelmann, S; Kühn, C
Infection and inflammation of the mammary gland,
and especially prevention of mastitis, are still major
challenges for the dairy industry. Different approaches
have been tried to reduce the incidence of mastitis.
Genetic selection of cows with lower susceptibility to
mastitis promises sustainable success in this regard.
Bos taurus autosome (BTA) 18, particularly the region
between 43 and 59 Mb, harbors quantitative trait
loci (QTL) for somatic cell score, a surrogate trait
for mastitis susceptibility. Scrutinizing the molecular
bases hereof, we challenged udders from half-sib heifers
having inherited either favorable paternal haplotypes
for somatic cell score (Q) or unfavorable haplotypes
(q) with the Staphylococcus aureus pathogen. RNA
sequencing was used for an in-depth analysis of challenge-
related alterations in the hepatic transcriptome.
Liver exerts highly relevant immune functions aside
from being the key metabolic organ. Hence, a holistic
approach focusing on the liver enabled us to identify
challenge-related and genotype-dependent differentially
expressed genes and underlying regulatory networks.
In response to the S. aureus challenge, we found that
heifers with Q haplotypes displayed more activated
immune genes and pathways after S. aureus challenge
compared with their q half-sibs. Furthermore, we found
a significant enrichment of differentially expressed loci
in the genomic target region on BTA18, suggesting the
existence of a regionally acting regulatory element with
effects on a variety of genes in this region.
2020-04-16T00:00:00Z
Elimination of Staphylococcus aureus from the bloodstream using a novel biomimetic sorbent haemoperfusion device.
Seffer, Malin-Theres
Eden, Gabriele
Engelmann, Susanne
Kielstein, Jan T
http://hdl.handle.net/10033/623189
2022-05-07T01:57:07Z
2020-08-24T00:00:00Z
Elimination of Staphylococcus aureus from the bloodstream using a novel biomimetic sorbent haemoperfusion device.
Seffer, Malin-Theres; Eden, Gabriele; Engelmann, Susanne; Kielstein, Jan T
Removal of bacteria from the blood by means of extracorporeal techniques has been attempted for decades. In late 2019, the European Union licensed the first ever haemoperfusion device for removal of bacteria from the blood. The active ingredient of Seraph 100 Microbind Affinity Blood Filter is ultrahigh molecular weight polyethylene beads with endpoint-attached heparin. Bacteria have been shown to bind to heparin as they would usually do to the heparan sulfate on the cell surface, thereby being removed from the blood stream. We describe the first case of a female chronic haemodialysis patient in which this device was clinically used for a Staphylococcus aureus infection that persisted for 4 days despite antibiotic therapy. After a single treatment, the bacterial load decreased and the blood cultures at the end of a 4 hour haemoperfusion exhibited no bacterial growth.
2020-08-24T00:00:00Z
Within-Host Adaptation of in a Bovine Mastitis Infection Is Associated with Increased Cytotoxicity.
Mayer, Katharina
Kucklick, Martin
Marbach, Helene
Ehling-Schulz, Monika
Engelmann, Susanne
Grunert, Tom
http://hdl.handle.net/10033/623032
2021-09-16T01:52:20Z
2021-08-17T00:00:00Z
Within-Host Adaptation of in a Bovine Mastitis Infection Is Associated with Increased Cytotoxicity.
Mayer, Katharina; Kucklick, Martin; Marbach, Helene; Ehling-Schulz, Monika; Engelmann, Susanne; Grunert, Tom
Within-host adaptation is a typical feature of chronic, persistent Staphylococcus aureus infections. Research projects addressing adaptive changes due to bacterial in-host evolution increase our understanding of the pathogen's strategies to survive and persist for a long time in various hosts such as human and bovine. In this study, we investigated the adaptive processes of S. aureus during chronic, persistent bovine mastitis using a previously isolated isogenic strain pair from a dairy cow with chronic, subclinical mastitis, in which the last variant (host-adapted, Sigma factor SigB-deficient) quickly replaced the initial, dominant variant. The strain pair was cultivated under specific in vitro infection-relevant growth-limiting conditions (iron-depleted RPMI under oxygen limitation). We used a combinatory approach of surfaceomics, molecular spectroscopic fingerprinting and in vitro phenotypic assays. Cellular cytotoxicity assays using red blood cells and bovine mammary epithelial cells (MAC-T) revealed changes towards a more cytotoxic phenotype in the host-adapted isolate with an increased alpha-hemolysin (α-toxin) secretion, suggesting an improved capacity to penetrate and disseminate the udder tissue. Our results foster the hypothesis that within-host evolved SigB-deficiency favours extracellular persistence in S. aureus infections. Here, we provide new insights into one possible adaptive strategy employed by S. aureus during chronic, bovine mastitis, and we emphasise the need to analyse genotype-phenotype associations under different infection-relevant growth conditions.
2021-08-17T00:00:00Z