Dept. Cell Biology (ZB)
http://hdl.handle.net/10033/6802
Abt. Zellbiologie (ZB)2024-03-28T18:51:23ZThe human otubain2-ubiquitin structure provides insights into the cleavage specificity of poly-ubiquitin-linkages.
http://hdl.handle.net/10033/346874
The human otubain2-ubiquitin structure provides insights into the cleavage specificity of poly-ubiquitin-linkages.
Altun, Mikael; Walter, Thomas S; Kramer, Holger B; Herr, Patrick; Iphöfer, Alexander; Boström, Johan; David, Yael; Komsany, Alia; Ternette, Nicola; Navon, Ami; Stuart, David I; Ren, Jingshan; Kessler, Benedikt M
Ovarian tumor domain containing proteases cleave ubiquitin (Ub) and ubiquitin-like polypeptides from proteins. Here we report the crystal structure of human otubain 2 (OTUB2) in complex with a ubiquitin-based covalent inhibitor, Ub-Br2. The ubiquitin binding mode is oriented differently to how viral otubains (vOTUs) bind ubiquitin/ISG15, and more similar to yeast and mammalian OTUs. In contrast to OTUB1 which has exclusive specificity towards Lys48 poly-ubiquitin chains, OTUB2 cleaves different poly-Ub linked chains. N-terminal tail swapping experiments between OTUB1 and OTUB2 revealed how the N-terminal structural motifs in OTUB1 contribute to modulating enzyme activity and Ub-chain selectivity, a trait not observed in OTUB2, supporting the notion that OTUB2 may affect a different spectrum of substrates in Ub-dependent pathways.
2015-01-01T00:00:00ZMinimal increase in genetic diversity enhances predation resistance.
http://hdl.handle.net/10033/278874
Minimal increase in genetic diversity enhances predation resistance.
Koh, Kai S; Matz, Carsten; Tan, Chuan H; LE, Hoang L; Rice, Scott A; Marshall, Dustin J; Steinberg, Peter D; Kjelleberg, Staffan
The importance of species diversity to emergent, ecological properties of communities is increasingly appreciated, but the importance of within-species genetic diversity for analogous emergent properties of populations is only just becoming apparent. Here, the properties and effects of genetic variation on predation resistance in populations were assessed and the molecular mechanism underlying these emergent effects was investigated. Using biofilms of the ubiquitous bacterium Serratia marcescens, we tested the importance of genetic diversity in defending biofilms against protozoan grazing, a main source of mortality for bacteria in all natural ecosystems. S. marcescens biofilms established from wild-type cells produce heritable, stable variants, which when experimentally combined, persist as a diverse assemblage and are significantly more resistant to grazing than either wild type or variant biofilms grown in monoculture. This diversity effect is biofilm-specific, a result of either facilitation or resource partitioning among variants, with equivalent experiments using planktonic cultures and grazers resulting in dominance by a single resistant strain. The variants studied are all the result of single nucleotide polymorphisms in one regulatory gene suggesting that the benefits of genetic diversity in clonal biofilms can occur through remarkably minimal genetic change. The findings presented here provide a new insight on the integration of genetics and population ecology, in which diversity arising through minimal changes in genotype can have major ecological implications for natural populations.
2012-04-01T00:00:00ZIn vitro field exposition of skin cells between 100 GHz and 2.52 THz
http://hdl.handle.net/10033/133572
In vitro field exposition of skin cells between 100 GHz and 2.52 THz
KLeine-Ostmann, Thomas; Jastrow, Christian; Salhi, Mohamed Amine; Schrader, Thorsten; Hintzsche, Henning; Stopper, Helga; Kärst, Uwe; Heinen, B.; Baaske, Kai; Koch, M.
2010-01-01T00:00:00ZUrinary collagen fragments are significantly altered in diabetes: a link to pathophysiology.
http://hdl.handle.net/10033/129760
Urinary collagen fragments are significantly altered in diabetes: a link to pathophysiology.
Maahs, David M; Siwy, Justyna; Argilés, Angel; Cerna, Marie; Delles, Christian; Dominiczak, Anna F; Gayrard, Nathalie; Iphöfer, Alexander; Jänsch, Lothar; Jerums, George; Medek, Karel; Mischak, Harald; Navis, Gerjan J; Roob, Johannes M; Rossing, Kasper; Rossing, Peter; Rychlík, Ivan; Schiffer, Eric; Schmieder, Roland E; Wascher, Thomas C; Winklhofer-Roob, Brigitte M; Zimmerli, Lukas U; Zürbig, Petra; Snell-Bergeon, Janet K
The pathogenesis of diabetes mellitus (DM) is variable, comprising different inflammatory and immune responses. Proteome analysis holds the promise of delivering insight into the pathophysiological changes associated with diabetes. Recently, we identified and validated urinary proteomics biomarkers for diabetes. Based on these initial findings, we aimed to further validate urinary proteomics biomarkers specific for diabetes in general, and particularity associated with either type 1 (T1D) or type 2 diabetes (T2D).
2010-01-01T00:00:00Z