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dc.contributor.authorCandela, Marco
dc.contributor.authorCentanni, Manuela
dc.contributor.authorFiori, Jessica
dc.contributor.authorBiagi, Elena
dc.contributor.authorTurroni, Silvia
dc.contributor.authorOrrico, Catia
dc.contributor.authorBergmann, Simone
dc.contributor.authorHammerschmidt, Sven
dc.contributor.authorBrigidi, Patrizia
dc.date.accessioned2010-09-14T10:50:07Z
dc.date.available2010-09-14T10:50:07Z
dc.date.issued2010-06
dc.identifier.citationDnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts. 2010, 156 (Pt 6):1609-18 Microbiology (Reading, Engl.)en
dc.identifier.issn1465-2080
dc.identifier.pmid20167618
dc.identifier.doi10.1099/mic.0.038307-0
dc.identifier.urihttp://hdl.handle.net/10033/111148
dc.description.abstractBifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strain B. animalis subsp. lactis BI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as a B. animalis subsp. lactis BI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure of B. animalis subsp. lactis BI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation of B. animalis subsp. lactis BI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host by B. animalis subsp. lactis BI07.
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBifidobacteriumen
dc.subject.meshBile Acids and Saltsen
dc.subject.meshGastrointestinal Tracten
dc.subject.meshHSP70 Heat-Shock Proteinsen
dc.subject.meshHumansen
dc.subject.meshMicroscopy, Electron, Transmissionen
dc.subject.meshPhosphopyruvate Hydrataseen
dc.subject.meshPlasminogenen
dc.subject.meshProteomeen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshUp-Regulationen
dc.titleDnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pharmaceutical Sciences, University of Bologna, Italy.en
dc.identifier.journalMicrobiology (Reading, England)en
refterms.dateFOA2012-06-15T00:00:00Z
html.description.abstractBifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strain B. animalis subsp. lactis BI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as a B. animalis subsp. lactis BI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure of B. animalis subsp. lactis BI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation of B. animalis subsp. lactis BI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host by B. animalis subsp. lactis BI07.


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