Global genotype-phenotype correlations in Pseudomonas aeruginosa.
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AbstractOnce the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.
CitationGlobal genotype-phenotype correlations in Pseudomonas aeruginosa. 2010, 6 (8) PLoS Pathog.
AffiliationChronic Pseudomonas Infections, Helmholtz Center for Infection Research, Braunschweig, Germany.
The following license files are associated with this item:
- An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants.
- Authors: Liberati NT, Urbach JM, Miyata S, Lee DG, Drenkard E, Wu G, Villanueva J, Wei T, Ausubel FM
- Issue date: 2006 Feb 21
- Genome-scale metabolic network analysis of the opportunistic pathogen Pseudomonas aeruginosa PAO1.
- Authors: Oberhardt MA, Puchałka J, Fryer KE, Martins dos Santos VA, Papin JA
- Issue date: 2008 Apr
- Comprehensive transposon mutant library of Pseudomonas aeruginosa.
- Authors: Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, Ernst S, Will O, Kaul R, Raymond C, Levy R, Chun-Rong L, Guenthner D, Bovee D, Olson MV, Manoil C
- Issue date: 2003 Nov 25
- Genotypic and phenotypic analysis of type III secretion system in a cohort of Pseudomonas aeruginosa bacteremia isolates: evidence for a possible association between O serotypes and exo genes.
- Authors: Berthelot P, Attree I, Plésiat P, Chabert J, de Bentzmann S, Pozzetto B, Grattard F, Groupe d'Etudes des Septicémies à Pseudomonas aeruginosa.
- Issue date: 2003 Aug 15
- Sequence diversity of Pseudomonas aeruginosa: impact on population structure and genome evolution.
- Authors: Kiewitz C, Tümmler B
- Issue date: 2000 Jun