Show simple item record

dc.contributor.authorMa, Bin*
dc.contributor.authorWinkelbach, Simon*
dc.contributor.authorLindenmaier, Werner*
dc.contributor.authorDittmar, Kurt E J*
dc.date.accessioned2007-06-20T07:44:01Z
dc.date.available2007-06-20T07:44:01Z
dc.date.issued2006
dc.identifier.citationActa Histochem. 2006, 108(4):243-57en
dc.identifier.issn0065-1281
dc.identifier.pmid16730369
dc.identifier.doi10.1016/j.acthis.2006.02.002
dc.identifier.urihttp://hdl.handle.net/10033/12399
dc.description.abstractMulti-colour imaging of immunofluorescently labelled tissue using confocal microscopy was accomplished by using colour addition theory. This new technique includes several improvements for immunolabelling: (1) the co-localization of two or more markers on one cell for the identification of specific cell populations; (2) the co-localization of two fluorescent dyes from secondary reagents for the identification of the cells; (3) a multi-step staining protocol with two primary antibodies originating from the same host species or with two or three biotin-conjugated primary antibodies. After image acquisition, colour segmentation/unmixing are applied to the single multi-colour image to generate multi-pseudo-channels for individual or co-localized fluorescent dyes. With this new technique, we have been able to visualize six cell populations simultaneously in the mouse lymph node and intestine. The efficiency of this method has also been demonstrated in the three-dimensional reconstruction of thick sections from mouse ileum. Our method is simple, efficient, and may be indispensable in experimental cell and tissue studies requiring multiple immunolabelling.
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent8221908 bytes
dc.format.extent7494900 bytes
dc.format.extent12518400 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent128063 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent-1 bytes
dc.format.extent346112 bytes
dc.format.extent724992 bytes
dc.format.mimetypeimage/tiff
dc.format.mimetypeapplication/pdf
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeapplication/postscript
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeimage/tiff
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/octet-stream
dc.format.mimetypeapplication/octet-stream
dc.language.isoenen
dc.titleSix-colour fluorescent imaging of lymphoid tissue based on colour addition theory.en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-05-23T12:10:51Z
html.description.abstractMulti-colour imaging of immunofluorescently labelled tissue using confocal microscopy was accomplished by using colour addition theory. This new technique includes several improvements for immunolabelling: (1) the co-localization of two or more markers on one cell for the identification of specific cell populations; (2) the co-localization of two fluorescent dyes from secondary reagents for the identification of the cells; (3) a multi-step staining protocol with two primary antibodies originating from the same host species or with two or three biotin-conjugated primary antibodies. After image acquisition, colour segmentation/unmixing are applied to the single multi-colour image to generate multi-pseudo-channels for individual or co-localized fluorescent dyes. With this new technique, we have been able to visualize six cell populations simultaneously in the mouse lymph node and intestine. The efficiency of this method has also been demonstrated in the three-dimensional reconstruction of thick sections from mouse ileum. Our method is simple, efficient, and may be indispensable in experimental cell and tissue studies requiring multiple immunolabelling.


Files in this item

Thumbnail
Name:
Publisher version
Thumbnail
Name:
Templatepage_plus doc_bma.pdf
Size:
-1bytes
Format:
PDF
Description:
main document
Thumbnail
Name:
Figure1.tif
Size:
7.841Mb
Format:
TIFF image
Description:
figure1
Thumbnail
Name:
Figure2.tif
Size:
7.147Mb
Format:
TIFF image
Description:
figure2
Thumbnail
Name:
Figure3.tif
Size:
-1bytes
Format:
TIFF image
Description:
figure3
Thumbnail
Name:
Figure4.tif
Size:
11.93Mb
Format:
TIFF image
Description:
figure4
Thumbnail
Name:
Figure5.tif
Size:
-1bytes
Format:
TIFF image
Description:
figure5
Thumbnail
Name:
Figure_6.eps
Size:
-1bytes
Format:
Postscript
Description:
figure6
Thumbnail
Name:
S Figure 1a.tif
Size:
-1bytes
Format:
TIFF image
Description:
Sfigure1a
Thumbnail
Name:
S Figure 1b.tif
Size:
125.0Kb
Format:
TIFF image
Description:
Sfigure1b
Thumbnail
Name:
S Figure 2a.tif
Size:
-1bytes
Format:
TIFF image
Description:
Sfigure2a
Thumbnail
Name:
S Figure 2b.tif
Size:
-1bytes
Format:
TIFF image
Description:
Sfigure2b
Thumbnail
Name:
Supplementary Table 1.doc
Size:
-1bytes
Format:
Microsoft Word
Description:
supplementary Table1
Thumbnail
Name:
Supplementary Table 2.doc
Size:
-1bytes
Format:
Microsoft Word
Description:
Supplementary Table2
Thumbnail
Name:
Table 1.doc
Size:
-1bytes
Format:
Microsoft Word
Description:
Table1
Thumbnail
Name:
Table 2.doc
Size:
-1bytes
Format:
Microsoft Word
Description:
Table2
Thumbnail
Name:
S video1.avi
Size:
338Kb
Format:
Unknown
Description:
Svideo1
Thumbnail
Name:
S video 2.avi
Size:
708Kb
Format:
Unknown
Description:
Svideo2

This item appears in the following Collection(s)

Show simple item record