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dc.contributor.authorDolowschiak, Tamas
dc.contributor.authorChassin, Cécilia
dc.contributor.authorBen Mkaddem, Sanae
dc.contributor.authorFuchs, Thilo M
dc.contributor.authorWeiss, Siegfried
dc.contributor.authorVandewalle, Alain
dc.contributor.authorHornef, Mathias W
dc.date.accessioned2011-05-17T13:40:40Z
dc.date.available2011-05-17T13:40:40Z
dc.date.issued2010
dc.identifier.citationPotentiation of epithelial innate host responses by intercellular communication. 2010, 6 (11):e1001194 PLoS Pathog.en
dc.identifier.issn1553-7374
dc.identifier.pmid21124989
dc.identifier.doi10.1371/journal.ppat.1001194
dc.identifier.urihttp://hdl.handle.net/10033/129685
dc.description.abstractThe epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Toxinsen
dc.subject.meshBlotting, Westernen
dc.subject.meshCell Adhesionen
dc.subject.meshCell Communicationen
dc.subject.meshCells, Cultureden
dc.subject.meshCytokinesen
dc.subject.meshEpithelial Cellsen
dc.subject.meshFlow Cytometryen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshHeat-Shock Proteinsen
dc.subject.meshHemolysin Proteinsen
dc.subject.meshImmunity, Innateen
dc.subject.meshImmunoblottingen
dc.subject.meshImmunoprecipitationen
dc.subject.meshIntestine, Smallen
dc.subject.meshListeria monocytogenesen
dc.subject.meshListeriosisen
dc.subject.meshMiceen
dc.subject.meshNADPH Oxidaseen
dc.subject.meshNitric Oxideen
dc.subject.meshRNA, Messengeren
dc.subject.meshReactive Oxygen Speciesen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.titlePotentiation of epithelial innate host responses by intercellular communication.en
dc.typeArticleen
dc.contributor.departmentHannover Medical School, Hannover, Germany.en
dc.identifier.journalPLoS pathogensen
refterms.dateFOA2018-06-13T05:26:11Z
html.description.abstractThe epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.


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