Interaction of metal oxide nanoparticles with lung surfactant protein A.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe alveolar lining fluid (ALF) covering the respiratory epithelium of the deep lung is the first biological barrier encountered by nanoparticles after inhalation. We here report for the first time significant differences for metal oxide nanoparticles to the binding of surfactant protein A (SP-A), the predominant protein component of ALF. SP-A is a physiologically most relevant protein and provides important biological signals. Also, it is involved in the lung's immune defence, controlling e.g. particle binding, uptake or transcytosis by epithelial cells and macrophages. In our study, we could prove different particle-protein interaction for eight different nanoparticles, whereas particles of the same bulk material revealed different adsorption patterns. In contrast to other proteins as bovine serum albumin (BSA), SP-A does not seem to significantly deagglomerate large agglomerates of particles, indicating different adsorption mechanisms as in the well-investigated model protein BSA. These findings may have important consequences for biological fate and toxicological effects of inhaled nanomaterials.
CitationInteraction of metal oxide nanoparticles with lung surfactant protein A. 2011, 77 (3):376-83 Eur J Pharm Biopharm
AffiliationDepartment of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany. email@example.com
JournalEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V
The following license files are associated with this item:
- Molecular adsorption at particle surfaces: a PM toxicity mediation mechanism.
- Authors: Kendall M, Brown L, Trought K
- Issue date: 2004
- Dispersion characteristics of various metal oxide secondary nanoparticles in culture medium for in vitro toxicology assessment.
- Authors: Kato H, Fujita K, Horie M, Suzuki M, Nakamura A, Endoh S, Yoshida Y, Iwahashi H, Takahashi K, Kinugasa S
- Issue date: 2010 Apr
- Change in agglomeration status and toxicokinetic fate of various nanoparticles in vivo following lung exposure in rats.
- Authors: Creutzenberg O, Bellmann B, Korolewitz R, Koch W, Mangelsdorf I, Tillmann T, Schaudien D
- Issue date: 2012 Oct
- Differential plasma protein binding to metal oxide nanoparticles.
- Authors: Deng ZJ, Mortimer G, Schiller T, Musumeci A, Martin D, Minchin RF
- Issue date: 2009 Nov 11
- Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A.
- Authors: Ruge CA, Kirch J, Cañadas O, Schneider M, Perez-Gil J, Schaefer UF, Casals C, Lehr CM
- Issue date: 2011 Dec