Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.
Supplementary Figure-1 Loessner.pdf
supplementary figure 1
Supplementary Figure-2 Loessner.pdf
supplementary figure 2
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractWe have used Salmonella enterica serovar Typhimurium (S. typhimurium) which are able to colonize tumours besides spleen and liver. Bacteria were equipped with constructs encoding green fluorescent protein or luciferase as reporters under control of the promoter PBAD that is inducible with L-arabinose. Reporter genes could be induced in culture but also when the bacteria resided within the mouse macrophages J774A.1. More important, strong expression of reporters by the bacteria could be detected in mice after administration of L-arabinose. This was especially pronounced in bacteria colonizing tumours. Histology demonstrated that the bacteria had accumulated in and close to necrotic areas of tumours. Bacterial gene induction was observed in both regions. PBAD is tightly controlled also in vivo because gene E of bacteriophage PhiX174 could be introduced as inducible suicide gene. The possibility to deliberately induce genes in bacterial carriers within the host should render them extremely powerful tools for tumour therapy.
CitationRemote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo. 2007, 9 (6):1529-37 Cell. Microbiol.
AffiliationMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. firstname.lastname@example.org
- Salmonella enterica Serovar Typhimurium HtrA: regulation of expression and role of the chaperone and protease activities during infection.
- Authors: Lewis C, Skovierova H, Rowley G, Rezuchova B, Homerova D, Stevenson A, Spencer J, Farn J, Kormanec J, Roberts M
- Issue date: 2009 Mar
- Use of in vivo-regulated promoters to deliver antigens from attenuated Salmonella enterica var. Typhimurium.
- Authors: Dunstan SJ, Simmons CP, Strugnell RA
- Issue date: 1999 Oct
- Targeted deletion of the ara operon of Salmonella typhimurium enhances L-arabinose accumulation and drives PBAD-promoted expression of anti-cancer toxins and imaging agents.
- Authors: Hong H, Lim D, Kim GJ, Park SH, Sik Kim H, Hong Y, Choy HE, Min JJ
- Issue date: 2014
- Synergistic antitumoral effects of human telomerase reverse transcriptase-mediated dual-apoptosis-related gene vector delivered by orally attenuated Salmonella enterica Serovar Typhimurium in murine tumor models.
- Authors: Fu W, Chu L, Han X, Liu X, Ren D
- Issue date: 2008 Jun
- Thermosensing coordinates a cis-regulatory module for transcriptional activation of the intracellular virulence system in Salmonella enterica serovar Typhimurium.
- Authors: Duong N, Osborne S, Bustamante VH, Tomljenovic AM, Puente JL, Coombes BK
- Issue date: 2007 Nov 23