Replication-deficient mutant Herpes Simplex Virus-1 targets professional antigen presenting cells and induces efficient CD4+ T helper responses.
dc.contributor.author | Fiorentini, Simona | |
dc.contributor.author | Marconi, Peggy | |
dc.contributor.author | Avolio, Manuela | |
dc.contributor.author | Marini, Elena | |
dc.contributor.author | Garrafa, Emirena | |
dc.contributor.author | Caracciolo, Sonia | |
dc.contributor.author | Rossi, Daniele | |
dc.contributor.author | Bozac, Alexandra | |
dc.contributor.author | Becker, Pablo D | |
dc.contributor.author | Gentili, Francesca | |
dc.contributor.author | Facchetti, Fabio | |
dc.contributor.author | Guzman, Carlos A | |
dc.contributor.author | Manservigi, Roberto | |
dc.contributor.author | Caruso, Arnaldo | |
dc.date.accessioned | 2008-03-05T09:45:09Z | |
dc.date.available | 2008-03-05T09:45:09Z | |
dc.date.issued | 2007-07 | |
dc.identifier.citation | Replication-deficient mutant Herpes Simplex Virus-1 targets professional antigen presenting cells and induces efficient CD4+ T helper responses. 2007, 9 (8):988-96 Microbes Infect. | en |
dc.identifier.issn | 1286-4579 | |
dc.identifier.pmid | 17553721 | |
dc.identifier.doi | 10.1016/j.micinf.2007.04.001 | |
dc.identifier.uri | http://hdl.handle.net/10033/19754 | |
dc.description.abstract | Both neutralizing antibodies and cytotoxic T-cells are necessary to control a viral infection. However, vigorous T helper responses are essential for their elicitation and maintenance. Here we show that a recombinant replication-deficient Herpes Simplex Virus (HSV)-1 vector encoding the Human Immunodeficiency Virus (HIV)-1 matrix protein p17 (T0-p17) was capable of infecting professional antigen presenting cells (APCs) in vitro and in vivo. The injection of T0-p17 in the mouse dermis generated a strong p17-specific CD4+ T helper response preceding both p17-specific humoral and effector T cell responses. Moreover, we show that T0-p17 infection did not interfere with the endogenous processing of the transgene encoded antigen, since infected APCs were able to evoke a strong recall response in vitro. Our results demonstrate that replication-deficient HSV vectors can be appealing candidates for the development of vaccines able to trigger T helper responses. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Antigen-Presenting Cells | en |
dc.subject.mesh | Antigens, CD4 | en |
dc.subject.mesh | CD4-Positive T-Lymphocytes | en |
dc.subject.mesh | Female | en |
dc.subject.mesh | Gene Products, gag | en |
dc.subject.mesh | Genetic Vectors | en |
dc.subject.mesh | HIV Antibodies | en |
dc.subject.mesh | HIV Antigens | en |
dc.subject.mesh | Herpesvirus 1, Human | en |
dc.subject.mesh | Humans | en |
dc.subject.mesh | Immunization | en |
dc.subject.mesh | Macrophages, Peritoneal | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Inbred BALB C | en |
dc.subject.mesh | Mutation | en |
dc.subject.mesh | Recombination, Genetic | en |
dc.subject.mesh | T-Lymphocytes, Helper-Inducer | en |
dc.subject.mesh | Viral Proteins | en |
dc.subject.mesh | Virus Replication | en |
dc.subject.mesh | gag Gene Products, Human Immunodeficiency Virus | en |
dc.title | Replication-deficient mutant Herpes Simplex Virus-1 targets professional antigen presenting cells and induces efficient CD4+ T helper responses. | en |
dc.type | Article | en |
dc.contributor.department | Department of Experimental and Applied Medicine, Section of Microbiology, University of Brescia Medical School, Piazzale Spedali Civili, 1, I-25123 Brescia, Italy. | en |
dc.identifier.journal | Microbes and infection / Institut Pasteur | en |
refterms.dateFOA | 2018-06-13T04:07:24Z | |
html.description.abstract | Both neutralizing antibodies and cytotoxic T-cells are necessary to control a viral infection. However, vigorous T helper responses are essential for their elicitation and maintenance. Here we show that a recombinant replication-deficient Herpes Simplex Virus (HSV)-1 vector encoding the Human Immunodeficiency Virus (HIV)-1 matrix protein p17 (T0-p17) was capable of infecting professional antigen presenting cells (APCs) in vitro and in vivo. The injection of T0-p17 in the mouse dermis generated a strong p17-specific CD4+ T helper response preceding both p17-specific humoral and effector T cell responses. Moreover, we show that T0-p17 infection did not interfere with the endogenous processing of the transgene encoded antigen, since infected APCs were able to evoke a strong recall response in vitro. Our results demonstrate that replication-deficient HSV vectors can be appealing candidates for the development of vaccines able to trigger T helper responses. |