Show simple item record

dc.contributor.authorBielecki, Piotr
dc.contributor.authorPuchałka, Jacek
dc.contributor.authorWos-Oxley, Melissa L
dc.contributor.authorLoessner, Holger
dc.contributor.authorGlik, Justyna
dc.contributor.authorKawecki, Marek
dc.contributor.authorNowak, Mariusz
dc.contributor.authorTümmler, Burkhard
dc.contributor.authorWeiss, Siegfried
dc.contributor.authordos Santos, Vítor A P Martins
dc.date.accessioned2012-02-24T13:36:59Z
dc.date.available2012-02-24T13:36:59Z
dc.date.issued2011
dc.identifier.citationIn-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs. 2011, 6 (9):e24235 PLoS ONEen
dc.identifier.issn1932-6203
dc.identifier.pmid21931663
dc.identifier.doi10.1371/journal.pone.0024235
dc.identifier.urihttp://hdl.handle.net/10033/212709
dc.description.abstractPseudomonas aeruginosa is a threatening, opportunistic pathogen causing disease in immunocompromised individuals. The hallmark of P. aeruginosa virulence is its multi-factorial and combinatorial nature. It renders such bacteria infectious for many organisms and it is often resistant to antibiotics. To gain insights into the physiology of P. aeruginosa during infection, we assessed the transcriptional programs of three different P. aeruginosa strains directly after isolation from burn wounds of humans. We compared the programs to those of the same strains using two infection models: a plant model, which consisted of the infection of the midrib of lettuce leaves, and a murine tumor model, which was obtained by infection of mice with an induced tumor in the abdomen. All control conditions of P. aeruginosa cells growing in suspension and as a biofilm were added to the analysis. We found that these different P. aeruginosa strains express a pool of distinct genetic traits that are activated under particular infection conditions regardless of their genetic variability. The knowledge herein generated will advance our understanding of P. aeruginosa virulence and provide valuable cues for the definition of prospective targets to develop novel intervention strategies.
dc.language.isoenen
dc.titleIn-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs.en
dc.typeArticleen
dc.contributor.departmentSystems and Synthetic Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.en
dc.identifier.journalPloS oneen
refterms.dateFOA2018-06-13T16:03:16Z
html.description.abstractPseudomonas aeruginosa is a threatening, opportunistic pathogen causing disease in immunocompromised individuals. The hallmark of P. aeruginosa virulence is its multi-factorial and combinatorial nature. It renders such bacteria infectious for many organisms and it is often resistant to antibiotics. To gain insights into the physiology of P. aeruginosa during infection, we assessed the transcriptional programs of three different P. aeruginosa strains directly after isolation from burn wounds of humans. We compared the programs to those of the same strains using two infection models: a plant model, which consisted of the infection of the midrib of lettuce leaves, and a murine tumor model, which was obtained by infection of mice with an induced tumor in the abdomen. All control conditions of P. aeruginosa cells growing in suspension and as a biofilm were added to the analysis. We found that these different P. aeruginosa strains express a pool of distinct genetic traits that are activated under particular infection conditions regardless of their genetic variability. The knowledge herein generated will advance our understanding of P. aeruginosa virulence and provide valuable cues for the definition of prospective targets to develop novel intervention strategies.


Files in this item

Thumbnail
Name:
Bielecki et al_final.pdf
Size:
1.431Mb
Format:
PDF
Description:
OPen Access publication

This item appears in the following Collection(s)

Show simple item record