Internalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authorsde Souza Carvalho, Cristiane
Gutierrez, Maximiliano Gabriel
MetadataShow full item record
AbstractBacterial and parasitic intracellular pathogens or their secreted products have been shown to induce host cell transcriptional responses, which may benefit the host, favour the microorganism or be unrelated to the infection. In most instances, however, it is not known if the host cell nucleus is proximately required for the development of an intracellular infection. This information can be obtained by the infection of artificially enucleated host cells (cytoplasts). This model, although rather extensively used in studies of viral infection, has only been applied to few bacterial pathogens, which do not include Mycobacterium spp. Here, we investigate the internalization, phagosome biogenesis and survival of M. smegmatis in enucleated type II alveolar epithelial cells. Cytoplasts were infected with M. smegmatis, but the percentage of infection was significantly lower than that of nucleated cells. Scanning electron microscopy indicated that in both cells and cytoplasts, bacteria were internalized by a phagocytosis-like mechanism. Interestingly, phagosome fusion with lysosomes and mycobacterial killing were both more efficient in enucleated than in nucleated cells, a finding that may be correlated with the increased number of autophagic vesicles developed in cytoplasts. We provide evidence that although quantitative changes were observed, the full development of the infection, as well as mycobacterial killing did not require the presence of the host cell nucleus.
CitationInternalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells. 2011, 13 (8):1234-49 Cell. Microbiol.
AffiliationDepartment of Vaccinology and Applied Microbiology, Research Group Phagosome Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- Dynamic life and death interactions between Mycobacterium smegmatis and J774 macrophages.
- Authors: Anes E, Peyron P, Staali L, Jordao L, Gutierrez MG, Kress H, Hagedorn M, Maridonneau-Parini I, Skinner MA, Wildeman AG, Kalamidas SA, Kuehnel M, Griffiths G
- Issue date: 2006 Jun
- Inhibition of phagosome-lysosome fusion in macrophages by certain mycobacteria can be explained by inhibition of lysosomal movements observed after phagocytosis.
- Authors: Hart PD, Young MR, Gordon AH, Sullivan KH
- Issue date: 1987 Oct 1
- On the killing of mycobacteria by macrophages.
- Authors: Jordao L, Bleck CK, Mayorga L, Griffiths G, Anes E
- Issue date: 2008 Feb
- Protein kinase G from pathogenic mycobacteria promotes survival within macrophages.
- Authors: Walburger A, Koul A, Ferrari G, Nguyen L, Prescianotto-Baschong C, Huygen K, Klebl B, Thompson C, Bacher G, Pieters J
- Issue date: 2004 Jun 18
- NF-kappa B activation controls phagolysosome fusion-mediated killing of mycobacteria by macrophages.
- Authors: Gutierrez MG, Mishra BB, Jordao L, Elliott E, Anes E, Griffiths G
- Issue date: 2008 Aug 15