Impact of glutamine transporters on pneumococcal fitness under infection-related conditions.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe genomic analysis of Streptococcus pneumoniae predicted six putative glutamine uptake systems, which are expressed under in vitro conditions, as shown here by reverse transcription-PCR. Four of these operons consist of glnHPQ, while two lack glnH, which encodes a soluble glutamine-binding protein. Here, we studied the impact of two of these glutamine ATP-binding cassette transporters on S. pneumoniae D39 virulence and phagocytosis, which consist of GlnQ and a translationally fused protein of GlnH and GlnP. Mice infected intranasally with D39Δgln0411/0412 showed significantly increased survival times and a significant delay in the development of pneumococcal pneumonia compared to those infected with D39, as observed in real time using bioluminescent pneumococci. In a mouse sepsis model, the mutant D39Δgln0411/0412 showed only moderate but significant attenuation. In contrast, the D39Δgln1098/1099 knockout strain was massively attenuated in the pneumonia and septicemia mouse infection model. To cause pneumonia or sepsis with D39Δgln1098/1099, infection doses 100- to 10,000-fold higher than those used for wild-type strain D39 were required. In an experimental mouse meningitis model, D39Δgln1098/1099 produced decreased levels of white blood cells in cerebrospinal fluid and showed decreased numbers of bacteria in the bloodstream compared to D39 and D39Δgln0411/0412. Phagocytosis experiments revealed significantly decreased intracellular survival rates of mutants D39Δgln1098/1099 and D39Δgln0411/0412 compared to wild-type D39, suggesting that the deficiency of Gln uptake systems impairs resistance to oxidative stress. Taken together, our results demonstrate that both glutamine uptake systems are required for full virulence of pneumococci but exhibit different impacts on the pathogenesis of pneumococci under in vivo conditions.
CitationImpact of glutamine transporters on pneumococcal fitness under infection-related conditions. 2011, 79 (1):44-58 Infect. Immun.
AffiliationDepartment of Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, Ernst Moritz Arndt Universität Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, D-17487 Greifswald, Germany.
JournalInfection and immunity
The following license files are associated with this item:
- The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence.
- Authors: Holmes AR, McNab R, Millsap KW, Rohde M, Hammerschmidt S, Mawdsley JL, Jenkinson HF
- Issue date: 2001 Sep
- Site-specific contributions of glutamine-dependent regulator GlnR and GlnR-regulated genes to virulence of Streptococcus pneumoniae.
- Authors: Hendriksen WT, Kloosterman TG, Bootsma HJ, Estevão S, de Groot R, Kuipers OP, Hermans PW
- Issue date: 2008 Mar
- Regulation of the arginine deiminase system by ArgR2 interferes with arginine metabolism and fitness of Streptococcus pneumoniae.
- Authors: Schulz C, Gierok P, Petruschka L, Lalk M, Mäder U, Hammerschmidt S
- Issue date: 2014 Dec 23
- Strain-specific impact of PsaR of Streptococcus pneumoniae on global gene expression and virulence.
- Authors: Hendriksen WT, Bootsma HJ, van Diepen A, Estevão S, Kuipers OP, de Groot R, Hermans PW
- Issue date: 2009 May
- The Ami-AliA/AliB permease of Streptococcus pneumoniae is involved in nasopharyngeal colonization but not in invasive disease.
- Authors: Kerr AR, Adrian PV, Estevão S, de Groot R, Alloing G, Claverys JP, Mitchell TJ, Hermans PW
- Issue date: 2004 Jul