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dc.contributor.authorReich, Uta
dc.contributor.authorFadeeva, Elena
dc.contributor.authorWarnecke, Athanasia
dc.contributor.authorPaasche, Gerrit
dc.contributor.authorMüller, Peter
dc.contributor.authorChichkov, Boris
dc.contributor.authorStöver, Timo
dc.contributor.authorLenarz, Thomas
dc.contributor.authorReuter, Günter
dc.date.accessioned2012-06-25T09:51:13Z
dc.date.available2012-06-25T09:51:13Z
dc.date.issued2012-05
dc.identifier.citationDirecting neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4):940-7 J. Biomed. Mater. Res. Part B Appl. Biomater.en_GB
dc.identifier.issn1552-4981
dc.identifier.pmid22287482
dc.identifier.doi10.1002/jbm.b.32656
dc.identifier.urihttp://hdl.handle.net/10033/230456
dc.description.abstractFor best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.
dc.language.isoenen
dc.rightsArchived with thanks to Journal of biomedical materials research. Part B, Applied biomaterialsen_GB
dc.titleDirecting neuronal cell growth on implant material surfaces by microstructuring.en
dc.typeArticleen
dc.contributor.departmentDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.en_GB
dc.identifier.journalJournal of biomedical materials research. Part B, Applied biomaterialsen_GB
refterms.dateFOA2013-05-15T00:00:00Z
html.description.abstractFor best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.


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