Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authorsvon Köckritz-Blickwede, Maren
MetadataShow full item record
AbstractThese days it has been increasingly recognized that mast cells (MCs) are critical components of host defense against pathogens. In this study, we have provided the first evidence that MCs can kill bacteria by entrapping them in extracellular structures similar to the extracellular traps described for neutrophils (NETs). We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs. Close contact of bacteria and MCs was required for full antimicrobial activity. Immunofluorescence and electron microscopy revealed that S pyogenes was entrapped by extracellular structures produced by MCs (MCETs), which are composed of DNA, histones, tryptase, and the antimicrobial peptide LL-37. Disruption of MCETs significantly reduced the antimicrobial effect of MCs, suggesting that intact extracellular webs are critical for effective inhibition of bacterial growth. Similar to NETs, production of MCETs was mediated by a reactive oxygen species (ROS)-dependent cell death mechanism accompanied by disruption of the nuclear envelope, which can be induced after stimulation of MCs with phorbol-12-myristate-13-acetate (PMA), H(2)O(2), or bacterial pathogens. Our study provides the first experimental evidence of antimicrobial extracellular traps formation by an immune cell population other than neutrophils.
CitationPhagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation. 2008, 111 (6):3070-80 Blood
AffiliationInfection Immunology Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany.
The following license files are associated with this item:
- Urticaria Neonatorum: accumulation of tryptase-expressing mast cells in the skin lesions of newborns with Erythema Toxicum.
- Authors: Nelson A, Ulfgren AK, Edner J, Ståbi B, Brismar H, Hultenby K, Marchini G
- Issue date: 2007 Dec
- Cutting edge: mast cell antimicrobial activity is mediated by expression of cathelicidin antimicrobial peptide.
- Authors: Di Nardo A, Vitiello A, Gallo RL
- Issue date: 2003 Mar 1
- Listeria monocytogenes induces mast cell extracellular traps.
- Authors: Campillo-Navarro M, Leyva-Paredes K, Donis-Maturano L, González-Jiménez M, Paredes-Vivas Y, Cerbulo-Vázquez A, Serafín-López J, García-Pérez B, Ullrich SE, Flores-Romo L, Pérez-Tapia SM, Estrada-Parra S, Estrada-García I, Chacón-Salinas R
- Issue date: 2017 Feb
- Neutrophil extracellular traps are induced by Mycobacterium tuberculosis.
- Authors: Ramos-Kichik V, Mondragón-Flores R, Mondragón-Castelán M, Gonzalez-Pozos S, Muñiz-Hernandez S, Rojas-Espinosa O, Chacón-Salinas R, Estrada-Parra S, Estrada-García I
- Issue date: 2009 Jan
- The antimicrobial peptides piscidins are stored in the granules of professional phagocytic granulocytes of fish and are delivered to the bacteria-containing phagosome upon phagocytosis.
- Authors: Mulero I, Noga EJ, Meseguer J, García-Ayala A, Mulero V
- Issue date: 2008