Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Lehr, C M
MetadataShow full item record
AbstractThere is increasing demand for automated cell reprogramming in the fields of cell biology, biotechnology and the biomedical sciences. Microfluidic-based platforms that provide unattended manipulation of adherent cells promise to be an appropriate basis for cell manipulation. In this study we developed a magnetically driven cell carrier to serve as a vehicle within an in vitro environment. To elucidate the impact of the carrier on cells, biocompatibility was estimated using the human adenocarcinoma cell line Caco-2. Besides evaluation of the quality of the magnetic carriers by field emission scanning electron microscopy, the rate of adherence, proliferation and differentiation of Caco-2 cells grown on the carriers was quantified. Moreover, the morphology of the cells was monitored by immunofluorescent staining. Early generations of the cell carrier suffered from release of cytotoxic nickel from the magnetic cushion. Biocompatibility was achieved by complete encapsulation of the nickel bulk within galvanic gold. The insulation process had to be developed stepwise and was controlled by parallel monitoring of the cell viability. The final carrier generation proved to be a proper support for cell manipulation, allowing proliferation of Caco-2 cells equal to that on glass or polystyrene as a reference for up to 10 days. Functional differentiation was enhanced by more than 30% compared with the reference. A flat, ferromagnetic and fully biocompatible carrier for cell manipulation was developed for application in microfluidic systems. Beyond that, this study offers advice for the development of magnetic cell carriers and the estimation of their biocompatibility.
CitationProcess optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation. 2012, 8 (3):1239-47 Acta Biomater
AffiliationDepartment of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
The following license files are associated with this item:
- Bridging the gap--biocompatibility of microelectronic materials.
- Authors: Bogner E, Dominizi K, Hagl P, Bertagnolli E, Wirth M, Gabor F, Brezna W, Wanzenboeck HD
- Issue date: 2006 Mar
- Influence of structured wafer surfaces on the characteristics of Caco-2 cells.
- Authors: Guell I, Wanzenboeck HD, Forouzan SS, Bertagnolli E, Bogner E, Gabor F, Wirth M
- Issue date: 2009 Jan
- Biocompatible, smooth, plasma-treated nickel-titanium surface--an adequate platform for cell growth.
- Authors: Chrzanowski W, Szade J, Hart AD, Knowles JC, Dalby MJ
- Issue date: 2012 Feb
- Cell response to magnetic glyconanoparticles: does the carbohydrate matter?
- Authors: de la Fuente JM, Alcántara D, Penadés S
- Issue date: 2007 Dec
- Bioeffects of moderate-intensity static magnetic fields on cell cultures.
- Authors: Dini L, Abbro L
- Issue date: 2005