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dc.contributor.authorVoss, Martin
dc.contributor.authorNimtz, Manfred
dc.contributor.authorLeimkühler, Silke
dc.date.accessioned2012-08-09T10:47:12Z
dc.date.available2012-08-09T10:47:12Z
dc.date.issued2011
dc.identifier.citationElucidation of the dual role of Mycobacterial MoeZR in molybdenum cofactor biosynthesis and cysteine biosynthesis. 2011, 6 (11):e28170 PLoS ONEen_GB
dc.identifier.issn1932-6203
dc.identifier.pmid22140533
dc.identifier.doi10.1371/journal.pone.0028170
dc.identifier.urihttp://hdl.handle.net/10033/237913
dc.description.abstractThe pathway of molybdenum cofactor biosynthesis has been studied in detail by using proteins from Mycobacterium species, which contain several homologs associated with the first steps of Moco biosynthesis. While all Mycobacteria species contain a MoeZR, only some strains have acquired an additional homolog, MoeBR, by horizontal gene transfer. The role of MoeBR and MoeZR was studied in detail for the interaction with the two MoaD-homologs involved in Moco biosynthesis, MoaD1 and MoaD2, in addition to the CysO protein involved in cysteine biosynthesis. We show that both proteins have a role in Moco biosynthesis, while only MoeZR, but not MoeBR, has an additional role in cysteine biosynthesis. MoeZR and MoeBR were able to complement an E. coli moeB mutant strain, but only in conjunction with the Mycobacterial MoaD1 or MoaD2 proteins. Both proteins were able to sulfurate MoaD1 and MoaD2 in vivo, while only MoeZR additionally transferred the sulfur to CysO. Our in vivo studies show that Mycobacteria have acquired several homologs to maintain Moco biosynthesis. MoeZR has a dual role in Moco- and cysteine biosynthesis and is involved in the sulfuration of MoaD and CysO, whereas MoeBR only has a role in Moco biosynthesis, which is not an essential function for Mycobacteria.
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAmino Acid Sequenceen_GB
dc.subject.meshBacterial Proteinsen_GB
dc.subject.meshCircular Dichroismen_GB
dc.subject.meshCoenzymesen_GB
dc.subject.meshCysteineen_GB
dc.subject.meshEscherichia colien_GB
dc.subject.meshGenes, Bacterialen_GB
dc.subject.meshGenetic Complementation Testen_GB
dc.subject.meshKineticsen_GB
dc.subject.meshMetalloproteinsen_GB
dc.subject.meshModels, Biologicalen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshMycobacteriumen_GB
dc.subject.meshNitrate Reductaseen_GB
dc.subject.meshPteridinesen_GB
dc.subject.meshSequence Homology, Amino Aciden_GB
dc.subject.meshSpectrometry, Mass, Electrospray Ionizationen_GB
dc.subject.meshSulfurtransferasesen_GB
dc.titleElucidation of the dual role of Mycobacterial MoeZR in molybdenum cofactor biosynthesis and cysteine biosynthesis.en
dc.typeArticleen
dc.contributor.departmentInstitute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany.en_GB
dc.identifier.journalPloS oneen_GB
refterms.dateFOA2018-06-13T02:30:15Z
html.description.abstractThe pathway of molybdenum cofactor biosynthesis has been studied in detail by using proteins from Mycobacterium species, which contain several homologs associated with the first steps of Moco biosynthesis. While all Mycobacteria species contain a MoeZR, only some strains have acquired an additional homolog, MoeBR, by horizontal gene transfer. The role of MoeBR and MoeZR was studied in detail for the interaction with the two MoaD-homologs involved in Moco biosynthesis, MoaD1 and MoaD2, in addition to the CysO protein involved in cysteine biosynthesis. We show that both proteins have a role in Moco biosynthesis, while only MoeZR, but not MoeBR, has an additional role in cysteine biosynthesis. MoeZR and MoeBR were able to complement an E. coli moeB mutant strain, but only in conjunction with the Mycobacterial MoaD1 or MoaD2 proteins. Both proteins were able to sulfurate MoaD1 and MoaD2 in vivo, while only MoeZR additionally transferred the sulfur to CysO. Our in vivo studies show that Mycobacteria have acquired several homologs to maintain Moco biosynthesis. MoeZR has a dual role in Moco- and cysteine biosynthesis and is involved in the sulfuration of MoaD and CysO, whereas MoeBR only has a role in Moco biosynthesis, which is not an essential function for Mycobacteria.


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