The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.
dc.contributor.author | Poblete-Castro, Ignacio | |
dc.contributor.author | Escapa, Isabel F | |
dc.contributor.author | Jäger, Christian | |
dc.contributor.author | Puchalka, Jacek | |
dc.contributor.author | Lam, Carolyn Ming Chi | |
dc.contributor.author | Schomburg, Dietmar | |
dc.contributor.author | Prieto, María Auxiliadora | |
dc.contributor.author | Martins dos Santos, Vítor A P | |
dc.date.accessioned | 2012-08-30T09:24:05Z | |
dc.date.available | 2012-08-30T09:24:05Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. 2012, 11:34 Microb. Cell Fact. | en_GB |
dc.identifier.issn | 1475-2859 | |
dc.identifier.pmid | 22433058 | |
dc.identifier.doi | 10.1186/1475-2859-11-34 | |
dc.identifier.uri | http://hdl.handle.net/10033/240591 | |
dc.description.abstract | Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures. | |
dc.language.iso | en | en |
dc.rights | Archived with thanks to Microbial cell factories | en_GB |
dc.subject.mesh | Biomass | en_GB |
dc.subject.mesh | Carbon | en_GB |
dc.subject.mesh | Energy Metabolism | en_GB |
dc.subject.mesh | Fatty Acids | en_GB |
dc.subject.mesh | Gene Expression Regulation, Bacterial | en_GB |
dc.subject.mesh | Metabolomics | en_GB |
dc.subject.mesh | Nitrogen | en_GB |
dc.subject.mesh | Polyhydroxyalkanoates | en_GB |
dc.subject.mesh | Proteomics | en_GB |
dc.subject.mesh | Pseudomonas putida | en_GB |
dc.subject.mesh | Transcriptome | en_GB |
dc.title | The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.de | en_GB |
dc.identifier.journal | Microbial cell factories | en_GB |
refterms.dateFOA | 2018-06-12T23:51:27Z | |
html.description.abstract | Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures. |