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dc.contributor.authorPoblete-Castro, Ignacio
dc.contributor.authorEscapa, Isabel F
dc.contributor.authorJäger, Christian
dc.contributor.authorPuchalka, Jacek
dc.contributor.authorLam, Carolyn Ming Chi
dc.contributor.authorSchomburg, Dietmar
dc.contributor.authorPrieto, María Auxiliadora
dc.contributor.authorMartins dos Santos, Vítor A P
dc.date.accessioned2012-08-30T09:24:05Z
dc.date.available2012-08-30T09:24:05Z
dc.date.issued2012
dc.identifier.citationThe metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. 2012, 11:34 Microb. Cell Fact.en_GB
dc.identifier.issn1475-2859
dc.identifier.pmid22433058
dc.identifier.doi10.1186/1475-2859-11-34
dc.identifier.urihttp://hdl.handle.net/10033/240591
dc.description.abstractPseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.
dc.language.isoenen
dc.rightsArchived with thanks to Microbial cell factoriesen_GB
dc.subject.meshBiomassen_GB
dc.subject.meshCarbonen_GB
dc.subject.meshEnergy Metabolismen_GB
dc.subject.meshFatty Acidsen_GB
dc.subject.meshGene Expression Regulation, Bacterialen_GB
dc.subject.meshMetabolomicsen_GB
dc.subject.meshNitrogenen_GB
dc.subject.meshPolyhydroxyalkanoatesen_GB
dc.subject.meshProteomicsen_GB
dc.subject.meshPseudomonas putidaen_GB
dc.subject.meshTranscriptomeen_GB
dc.titleThe metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.deen_GB
dc.identifier.journalMicrobial cell factoriesen_GB
refterms.dateFOA2018-06-12T23:51:27Z
html.description.abstractPseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.


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