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dc.contributor.authorWargenau, Andreasen_GB
dc.contributor.authorFleissner, Andréen_GB
dc.contributor.authorBolten, Christoph Josefen_GB
dc.contributor.authorRohde, Manfreden_GB
dc.contributor.authorKampen, Ingoen_GB
dc.contributor.authorKwade, Arnoen_GB
dc.date.accessioned2012-08-31T14:35:10Z
dc.date.available2012-08-31T14:35:10Z
dc.date.issued2011-12
dc.identifier.citationOn the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments. 2011, 162 (10):1011-7 Res. Microbiol.en_GB
dc.identifier.issn1769-7123
dc.identifier.pmid21835241
dc.identifier.doi10.1016/j.resmic.2011.07.006
dc.identifier.urihttp://hdl.handle.net/10033/240838
dc.description.abstractThe electrostatic surface potential of fungal spores is generally regarded as potentially influencing spore aggregation and pellet formation in submerged cultures of filamentous fungi. Spores of Aspergillus niger are typically characterized by negative zeta potentials over a wide range of pH values. In this study, this particular behavior is ascribed to the presence of an extensive melanin coating. It is proposed on the basis of zeta potential and pigment extraction experiments that this outermost layer affects the pH-dependent surface potential in two manners: (i) by the addition of negative charges to the spore surface and (ii) by the pH-dependent release of melanin pigment. Chemical analyses revealed that deprotonation of melanin-bound carboxyl groups is most probably responsible for pigment release under acidic conditions. These findings were incorporated into a simple model which has the ability to qualitatively explain the results of zeta potential experiments and, moreover, to provide the basis for quantitative investigations on the role of electrostatics in spore aggregation.
dc.language.isoenen
dc.rightsArchived with thanks to Research in microbiologyen_GB
dc.subject.meshAspergillus nigeren_GB
dc.subject.meshEnvironmenten_GB
dc.subject.meshHydrogen-Ion Concentrationen_GB
dc.subject.meshMelaninsen_GB
dc.subject.meshMicroscopy, Electron, Transmissionen_GB
dc.subject.meshSpores, Fungalen_GB
dc.subject.meshStatic Electricityen_GB
dc.subject.meshSurface Propertiesen_GB
dc.titleOn the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments.en
dc.typeArticleen
dc.contributor.departmentInstitut für Partikeltechnik, Technische Universität Braunschweig, Volkmaroder Straße 5, D-38104 Braunschweig, Germany. wargenau@a-wargenau.deen_GB
dc.identifier.journalResearch in microbiologyen_GB
refterms.dateFOA2018-06-13T03:55:49Z
html.description.abstractThe electrostatic surface potential of fungal spores is generally regarded as potentially influencing spore aggregation and pellet formation in submerged cultures of filamentous fungi. Spores of Aspergillus niger are typically characterized by negative zeta potentials over a wide range of pH values. In this study, this particular behavior is ascribed to the presence of an extensive melanin coating. It is proposed on the basis of zeta potential and pigment extraction experiments that this outermost layer affects the pH-dependent surface potential in two manners: (i) by the addition of negative charges to the spore surface and (ii) by the pH-dependent release of melanin pigment. Chemical analyses revealed that deprotonation of melanin-bound carboxyl groups is most probably responsible for pigment release under acidic conditions. These findings were incorporated into a simple model which has the ability to qualitatively explain the results of zeta potential experiments and, moreover, to provide the basis for quantitative investigations on the role of electrostatics in spore aggregation.


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