Genome-wide localization and expression profiling establish Sp2 as a sequence-specific transcription factor regulating vitally important genes.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe transcription factor Sp2 is essential for early mouse development and for proliferation of mouse embryonic fibroblasts in culture. Yet its mechanisms of action and its target genes are largely unknown. In this study, we have combined RNA interference, in vitro DNA binding, chromatin immunoprecipitation sequencing and global gene-expression profiling to investigate the role of Sp2 for cellular functions, to define target sites and to identify genes regulated by Sp2. We show that Sp2 is important for cellular proliferation that it binds to GC-boxes and occupies proximal promoters of genes essential for vital cellular processes including gene expression, replication, metabolism and signalling. Moreover, we identified important key target genes and cellular pathways that are directly regulated by Sp2. Most significantly, Sp2 binds and activates numerous sequence-specific transcription factor and co-activator genes, and represses the whole battery of cholesterol synthesis genes. Our results establish Sp2 as a sequence-specific regulator of vitally important genes.
CitationGenome-wide localization and expression profiling establish Sp2 as a sequence-specific transcription factor regulating vitally important genes. 2012, 40 (16):7844-57 Nucleic Acids Res.
AffiliationInstitute of Molecular Biology and Tumor Research, Philipps-University, Emil-Mannkopff-Str. 2, D-35032 Marburg, Germany.
JournalNucleic acids research
The following license files are associated with this item:
- Zinc finger independent genome-wide binding of Sp2 potentiates recruitment of histone-fold protein Nf-y distinguishing it from Sp1 and Sp3.
- Authors: Völkel S, Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G
- Issue date: 2015 Mar
- Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression.
- Authors: Kingsley C, Winoto A
- Issue date: 1992 Oct
- The gonadotropin-regulated long-chain acyl CoA synthetase gene: a novel downstream Sp1/Sp3 binding element critical for transcriptional promoter activity.
- Authors: Sheng Y, Li J, Dufau ML, Tsai-Morris CH
- Issue date: 2005 Oct 24
- Functional significance of Sp1, Sp2, and Sp3 transcription factors in regulation of the murine CTP:phosphocholine cytidylyltransferase alpha promoter.
- Authors: Bakovic M, Waite KA, Vance DE
- Issue date: 2000 Apr
- Sp2 DNA binding activity and trans-activation are negatively regulated in mammalian cells.
- Authors: Moorefield KS, Fry SJ, Horowitz JM
- Issue date: 2004 Apr 2