Impact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Weber, Andreas P M
Mendel, Ralf R
MetadataShow full item record
AbstractHigh concentrations of sulfur dioxide (SO(2) ) as an air pollutant, and its derivative sulfite, cause abiotic stress that can lead to cell death. It is currently unknown to what extent plant fumigation triggers specific transcriptional responses. To address this question, and to test the hypothesis that sulfite oxidase (SO) is acting in SO(2) detoxification, we compared Arabidopsis wildtype (WT) and SO knockout lines (SO-KO) facing the impact of 600 nl l(-1) SO(2) , using RNAseq to quantify absolute transcript abundances. These transcriptome data were correlated to sulfur metabolism-related enzyme activities and metabolites obtained from identical samples in a previous study. SO-KO plants exhibited remarkable and broad regulative responses at the mRNA level, especially in transcripts related to sulfur metabolism enzymes, but also in those related to stress response and senescence. Focusing on SO regulation, no alterations were detectable in the WT, whereas in SO-KO plants we found up-regulation of two splice variants of the SO gene, although this gene is not functional in this line. Our data provide evidence for the highly specific coregulation between SO and sulfur-related enzymes like APS reductase, and suggest two novel candidates for involvement in SO(2) detoxification: an apoplastic peroxidase, and defensins as putative cysteine mass storages.
CitationImpact of SO(2) on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing. 2012, 196 (4):1074-85 New Phytol.
AffiliationInstitut für Pflanzenbiologie, Technische Universität Braunschweig, Braunschweig, Germany.
JournalThe New phytologist
The following license files are associated with this item:
- Sulfite oxidase controls sulfur metabolism under SO2 exposure in Arabidopsis thaliana.
- Authors: Randewig D, Hamisch D, Herschbach C, Eiblmeier M, Gehl C, Jurgeleit J, Skerra J, Mendel RR, Rennenberg H, Hänsch R
- Issue date: 2012 Jan
- Genome-wide transcriptome analysis of Arabidopsis response to sulfur dioxide fumigation.
- Authors: Zhao J, Yi H
- Issue date: 2014 Oct
- Isolation and functional analysis of sulfite oxidase gene AtSO promoter from Arabidopsis thaliana.
- Authors: Xia ZL, Wang MP, Liu QJ
- Issue date: 2007 Oct
- Sulfite oxidase protects plants against sulfur dioxide toxicity.
- Authors: Brychkova G, Xia Z, Yang G, Yesbergenova Z, Zhang Z, Davydov O, Fluhr R, Sagi M
- Issue date: 2007 May
- Sulphite oxidase as key enzyme for protecting plants against sulphur dioxide.
- Authors: Lang C, Popko J, Wirtz M, Hell R, Herschbach C, Kreuzwieser J, Rennenberg H, Mendel RR, Hänsch R
- Issue date: 2007 Apr