Show simple item record

dc.contributor.authorOnder, Lucas
dc.contributor.authorScandella, Elke
dc.contributor.authorChai, Qian
dc.contributor.authorFirner, Sonja
dc.contributor.authorMayer, Christian T
dc.contributor.authorSparwasser, Tim
dc.contributor.authorThiel, Volker
dc.contributor.authorRülicke, Thomas
dc.contributor.authorLudewig, Burkhard
dc.date.accessioned2013-03-05T15:15:47Zen
dc.date.available2013-03-05T15:15:47Zen
dc.date.issued2011en
dc.identifier.citationA novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo. 2011, 2:50 Front Immunolen_GB
dc.identifier.issn1664-3224en
dc.identifier.pmid22566840en
dc.identifier.doi10.3389/fimmu.2011.00050en
dc.identifier.urihttp://hdl.handle.net/10033/271194en
dc.description.abstractStromal cells provide the structural foundation of secondary lymphoid organs (SLOs), and regulate leukocyte access and cell migration within the different compartments of spleen and lymph nodes (LNs). Furthermore, several stromal cell subsets have been implied in shaping of T cell responses through direct presentation of antigen. Despite significant gain of knowledge on the biology of different SLO-resident stromal cell subsets, their molecular and functional characterization has remained incomplete. To address this need, we have generated a bacterial artificial chromosome-transgenic mouse model that utilizes the podoplanin (pdpn) promoter to express the Cre-recombinase exclusively in stromal cells of SLOs. The characterization of the Pdpn-Cre mouse revealed transgene expression in subsets of fibroblastic reticular cells and lymphatic endothelial cells in LNs. Furthermore, the transgene facilitated the identification of a novel splenic perivascular stromal cell subpopulation that forms web-like structures around central arterioles. Assessment of the in vivo antigen expression in the genetically tagged stromal cells in Pdpn-Cre mice revealed activation of both MHC I and II-restricted TCR transgenic T cells. Taken together, stromal pdpn-Cre expression is well-suited to characterize the phenotype and to dissect the function of lymphoid organ stromal cells.
dc.language.isoenen
dc.rightsArchived with thanks to Frontiers in immunologyen_GB
dc.titleA novel bacterial artificial chromosome-transgenic podoplanin-cre mouse targets lymphoid organ stromal cells in vivo.en
dc.typeArticleen
dc.contributor.departmentInstitute of Immunobiology, Cantonal Hospital St. Gallen St. Gallen, Switzerland.en_GB
dc.identifier.journalFrontiers in immunologyen_GB
refterms.dateFOA2018-06-12T21:52:53Z
html.description.abstractStromal cells provide the structural foundation of secondary lymphoid organs (SLOs), and regulate leukocyte access and cell migration within the different compartments of spleen and lymph nodes (LNs). Furthermore, several stromal cell subsets have been implied in shaping of T cell responses through direct presentation of antigen. Despite significant gain of knowledge on the biology of different SLO-resident stromal cell subsets, their molecular and functional characterization has remained incomplete. To address this need, we have generated a bacterial artificial chromosome-transgenic mouse model that utilizes the podoplanin (pdpn) promoter to express the Cre-recombinase exclusively in stromal cells of SLOs. The characterization of the Pdpn-Cre mouse revealed transgene expression in subsets of fibroblastic reticular cells and lymphatic endothelial cells in LNs. Furthermore, the transgene facilitated the identification of a novel splenic perivascular stromal cell subpopulation that forms web-like structures around central arterioles. Assessment of the in vivo antigen expression in the genetically tagged stromal cells in Pdpn-Cre mice revealed activation of both MHC I and II-restricted TCR transgenic T cells. Taken together, stromal pdpn-Cre expression is well-suited to characterize the phenotype and to dissect the function of lymphoid organ stromal cells.


Files in this item

Thumbnail
Name:
Onder et al_final.pdf
Size:
1.259Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record