Arranged sevenfold: structural insights into the C-terminal oligomerization domain of human C4b-binding protein.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Degiacomi, Matteo T
Dal Peraro, Matteo
van den Heuvel, Joop
Heinz, Dirk W
MetadataShow full item record
AbstractThe complement system as a major part of innate immunity is the first line of defense against invading microorganisms. Orchestrated by more than 60 proteins, its major task is to discriminate between host cells and pathogens and to initiate immune response. Additional recognition of necrotic or apoptotic cells demands a fine-tune regulation of this powerful system. C4b-binding protein (C4BP) is the major inhibitor of the classical complement and lectin pathway. The crystal structure of the human C4BP oligomerization domain in its 7α isoform and molecular simulations provide first structural insights of C4BP oligomerization. The heptameric core structure is stabilized by intermolecular disulfide bonds. In addition, thermal shift assays indicate that layers of electrostatic interactions mainly contribute to the extraordinary thermodynamic stability of the complex. These findings make C4BP a promising scaffold for multivalent ligand display with applications in immunology and biological chemistry.
CitationArranged sevenfold: structural insights into the C-terminal oligomerization domain of human C4b-binding protein. 2013, 425 (8):1302-17 J. Mol. Biol.
AffiliationInstitute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Petersenstraße 22, 64287 Darmstadt, Germany.
JournalJournal of molecular biology
The following license files are associated with this item:
- Solid-state NMR sequential assignments of the C-terminal oligomerization domain of human C4b-binding protein.
- Authors: Luckgei N, Habenstein B, Ravotti F, Megy S, Penin F, Marchand JB, Hill F, Böckmann A, Meier BH
- Issue date: 2014 Apr
- Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein.
- Authors: Sjöberg AP, Trouw LA, McGrath FD, Hack CE, Blom AM
- Issue date: 2006 Jun 15
- Complement inhibitor C4b-binding protein-friend or foe in the innate immune system?
- Authors: Blom AM, Villoutreix BO, Dahlbäck B
- Issue date: 2004 Apr
- Human C4b-binding protein, structural basis for interaction with streptococcal M protein, a major bacterial virulence factor.
- Authors: Jenkins HT, Mark L, Ball G, Persson J, Lindahl G, Uhrin D, Blom AM, Barlow PN
- Issue date: 2006 Feb 10