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dc.contributor.authorSchwerk, Johannes
dc.contributor.authorKöster, Mario
dc.contributor.authorHauser, Hansjörg
dc.contributor.authorRohde, Manfred
dc.contributor.authorFulde, Marcus
dc.contributor.authorHornef, Mathias W
dc.contributor.authorMay, Tobias
dc.date.accessioned2013-09-20T12:05:26Zen
dc.date.available2013-09-20T12:05:26Zen
dc.date.issued2013en
dc.identifier.citationGeneration of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions. 2013, 8 (8):e72700 PLoS ONEen_GB
dc.identifier.issn1932-6203en
dc.identifier.pmid23940817en
dc.identifier.doi10.1371/journal.pone.0072700en
dc.identifier.urihttp://hdl.handle.net/10033/301998en
dc.description.abstractCell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.titleGeneration of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions.en
dc.typeArticleen
dc.contributor.departmentDepartment of Gene Regulation and Differentiation, Helmholtz Centre for Infection Research, Braunschweig, Germany.en_GB
dc.identifier.journalPloS oneen_GB
refterms.dateFOA2018-06-13T14:08:46Z
html.description.abstractCell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.


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