Improved production of medium-chain-length Polyhydroxyalkanotes in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida strains.
Name:
Poblete-Castro et al_final.pdf
Size:
1.006Mb
Format:
PDF
Description:
Open Access publication
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
2013-10-22
Metadata
Show full item recordAbstract
One of the major challenges in metabolic engineering for enhanced synthesis of value-added chemicals is to design and develop new strains which can be translated into well-controlled fermentation processes using bioreactors. The aim of this study was to assess the influence of various fed-batch strategies in the performance of metabolically-engineered Pseudomonas putida strains, ∆gcd and ∆gcd-pgl, for improving production of medium-chain-length poly-hydroxyalkanoates (mcl-PHAs) using glucose as the only carbon source. First we developed a fed-batch process which comprised an initial phase of biomass accumulation based on an exponential feeding carbon-limited strategy. For the mcl-PHA accumulation stage, three induction techniques were tested under nitrogen limitation. The substrate-pulse feeding was more efficient than the constant-feeding approach to promote the accumulation of the desirable product. Nonetheless, the most efficient approach for maximum PHA synthesis was the application of a dissolved-oxygen-stat feeding strategy (DO-stat), where P. putida ∆gcd mutant strain showed a final PHA content and specific PHA productivity of 67%, and 0.83 [g•L(-1)•h(-1)], respectively. To our knowledge this mcl-PHA titer is the highest value that has been ever reported using glucose as the solely carbon and energy source. Our results also highlighted the effect of different fed-batch strategies upon the extent of realization of the intended metabolic modification of the mutant strains.Citation
Improved production of medium-chain-length Polyhydroxyalkanotes in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida strains. 2013: J. Microbiol. Biotechnol.Affiliation
Microbial Drugs Group, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.PubMed ID
24150495Type
ArticleISSN
1738-8872The following license files are associated with this item:
Related articles
- Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida.
- Authors: Borrero-de Acuña JM, Bielecka A, Häussler S, Schobert M, Jahn M, Wittmann C, Jahn D, Poblete-Castro I
- Issue date: 2014 Jun 19
- Enhanced yield of medium-chain-length polyhydroxyalkanoates from nonanoic acid by co-feeding glucose in carbon-limited, fed-batch culture.
- Authors: Sun Z, Ramsay J, Guay M, Ramsay B
- Issue date: 2009 Sep 25
- Fed-batch production of unsaturated medium-chain-length polyhydroxyalkanoates with controlled composition by Pseudomonas putida KT2440.
- Authors: Sun Z, Ramsay JA, Guay M, Ramsay BA
- Issue date: 2009 Mar
- Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.
- Authors: Beckers V, Poblete-Castro I, Tomasch J, Wittmann C
- Issue date: 2016 May 3
- Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440.
- Authors: Sun Z, Ramsay JA, Guay M, Ramsay BA
- Issue date: 2007 Feb