Analytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS.
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AbstractPurification and quality control of therapeutic peptides is often performed by one single method, RP-HPLC. As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel. The basic therapeutic peptide aviptadil served as a model substance to study the impurity profiles revealing 15 detectable impurities using CE-MS, two were detected by an appropriate nanoRP-HPLC-MS method. None of the impurities detected by CE were observed in LC and vice versa. The LOD in CE-MS was determined in the base peak electropherogram at ∼1fmol, a value 2500 times smaller than the LOD found in nanoRP-HPLC-MS (3pmol). In nanoRP-HPLC-MS only 0.2% of the extrapolated CE-MS signal for a 25ng aviptadil load was observed. We conclude that both, the LOD as well as the impurity profile of aviptadil, as analyzed by nanoRP-HPLC are influenced by both, the ligand-derivatized silica matrix and the flow-rate. Peptides may disappear completely and their variable emergence may lead to the determination of incorrect ratios as present in the sample.
CitationAnalytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS. 2014, 88:477-82 J Pharm Biomed Anal
AffiliationBiotech Processes and Analytics Department, PharmBioTec GmbH, D-66123 Saarbrücken, Germany. Electronic address: firstname.lastname@example.org.
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- Optimization and evaluation of a sheathless capillary electrophoresis-electrospray ionization mass spectrometry platform for peptide analysis: comparison to liquid chromatography-electrospray ionization mass spectrometry.
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- Issue date: 2011 Oct 1
- On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification.
- Authors: Janini GM, Zhou M, Yu LR, Blonder J, Gignac M, Conrads TP, Issaq HJ, Veenstra TD
- Issue date: 2003 Nov 1
- Forced degradation and impurity profiling: recent trends in analytical perspectives.
- Authors: Jain D, Basniwal PK
- Issue date: 2013 Dec
- Capillary electrophoresis-mass spectrometry in impurity profiling of pharmaceutical products.
- Authors: Visky D, Jimidar I, Van Ael W, Vennekens T, Redlich D, De Smet M
- Issue date: 2005 Apr
- Design and performance of a sheathless capillary electrophoresis/mass spectrometry interface by combining fused-silica capillaries with gold-coated nanoelectrospray tips.
- Authors: Kele Z, Ferenc G, Klement E, Tóth GK, Janáky T
- Issue date: 2005