A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC.
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AbstractIn this study we investigated the influence of the global response regulator PhoP on the complex regulatory cascade controlling expression of early stage virulence genes of Yersinia pseudotuberculosis via the virulence regulator RovA. Our analysis revealed the following novel features: (1) PhoP activates expression of the CsrC RNA in Y. pseudotuberculosis, leading to activation of RovA synthesis through the CsrABC-RovM cascade, (2) activation of csrC transcription is direct and PhoP is shown to bind to two separate PhoP box-like sites, (3) PhoP-mediated activation results in transcription from two different promoters closely downstream of the PhoP binding sites, leading to two distinct CsrC RNAs, and (4) the stability of the CsrC RNAs differs significantly between the Y. pseudotuberculosis strains YPIII and IP32953 due to a 20 nucleotides insertion in CsrC(IP32953), which renders the transcript more susceptible to degradation. In summary, our study showed that PhoP-mediated influence on the regulatory cascade controlling the Csr system and RovA in Y. pseudotuberculosis varies within the species, suggesting that the Csr system is a focal point to readjust and adapt the genus to different hosts and reservoirs.
CitationA direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC. 2014, 11 (5):580-93 RNA Biol
AffiliationDepartment of Molecular Infection Biology; Helmholtz Centre for Infection Research; Braunschweig, Germany.
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- A Csr-type regulatory system, including small non-coding RNAs, regulates the global virulence regulator RovA of Yersinia pseudotuberculosis through RovM.
- Authors: Heroven AK, Böhme K, Rohde M, Dersch P
- Issue date: 2008 Jun
- RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis.
- Authors: Heroven AK, Dersch P
- Issue date: 2006 Dec
- The role of the phoPQ operon in the pathogenesis of the fully virulent CO92 strain of Yersinia pestis and the IP32953 strain of Yersinia pseudotuberculosis.
- Authors: Bozue J, Mou S, Moody KL, Cote CK, Trevino S, Fritz D, Worsham P
- Issue date: 2011 Jun
- The response regulator PhoP of Yersinia pseudotuberculosis is important for replication in macrophages and for virulence.
- Authors: Grabenstein JP, Marceau M, Pujol C, Simonet M, Bliska JB
- Issue date: 2004 Sep
- Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis.
- Authors: Zhang Y, Gao H, Wang L, Xiao X, Tan Y, Guo Z, Zhou D, Yang R
- Issue date: 2011