The arginine-ornithine antiporter ArcD contributes to biological fitness of Streptococcus suis.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe arginine-ornithine antiporter (ArcD) is part of the Arginine Deiminase System (ADS), a catabolic, energy-providing pathway found in a variety of different bacterial species, including the porcine zoonotic pathogen Streptococcus suis. The ADS has recently been shown to play a role in the pathogenicity of S. suis, in particular in its survival in host cells. The contribution of arginine and arginine transport mediated by ArcD, however, has yet to be clarified. In the present study, we showed by experiments using [U-(13)C6]arginine as a tracer molecule that S. suis is auxotrophic for arginine and that bacterial growth depends on the uptake of extracellular arginine. To further study the role of ArcD in arginine metabolism, we generated an arcD-specific mutant strain and characterized its growth compared to the wild-type (WT) strain, a virulent serotype 2 strain. The mutant strain showed a markedly reduced growth in chemically defined media supplemented with arginine when compared to the WT strain, suggesting that ArcD promotes arginine uptake. To further evaluate the in vivo relevance of ArcD, we studied the intracellular bacterial survival of the arcD mutant strain in an epithelial cell culture infection model. The mutant strain was substantially attenuated, and its reduced intracellular survival rate correlated with a lower ability to neutralize the acidified environment. Based on these results, we propose that ArcD, by its function as an arginine-ornithine antiporter, is important for supplying arginine as substrate of the ADS and, thereby, contributes to biological fitness and virulence of S. suis in the host.
CitationThe arginine-ornithine antiporter ArcD contributes to biological fitness of Streptococcus suis. 2014, 4:107 Front Cell Infect Microbiol
AffiliationDepartment of Infectious Diseases, Institute for Microbiology, University of Veterinary Medicine Hannover, Germany ; Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI) Braunschweig, Germany.
The following license files are associated with this item:
- Arginine-Ornithine Antiporter ArcD Controls Arginine Metabolism and Interspecies Biofilm Development of Streptococcus gordonii.
- Authors: Sakanaka A, Kuboniwa M, Takeuchi H, Hashino E, Amano A
- Issue date: 2015 Aug 28
- Regulation of the arginine deiminase system by ArgR2 interferes with arginine metabolism and fitness of Streptococcus pneumoniae.
- Authors: Schulz C, Gierok P, Petruschka L, Lalk M, Mäder U, Hammerschmidt S
- Issue date: 2014 Dec 23
- Structure, regulation, and putative function of the arginine deiminase system of Streptococcus suis.
- Authors: Gruening P, Fulde M, Valentin-Weigand P, Goethe R
- Issue date: 2006 Jan
- Convergent evolution of the arginine deiminase pathway: the ArcD and ArcE arginine/ornithine exchangers.
- Authors: Noens EE, Lolkema JS
- Issue date: 2017 Feb
- arcD, the first gene of the arc operon for anaerobic arginine catabolism in Pseudomonas aeruginosa, encodes an arginine-ornithine exchanger.
- Authors: Verhoogt HJ, Smit H, Abee T, Gamper M, Driessen AJ, Haas D, Konings WN
- Issue date: 1992 Mar