The Mycobacterium avium ssp. paratuberculosis specific mptD gene is required for maintenance of the metabolic homeostasis necessary for full virulence in mouse infections.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Oelemann, Walter M R
MetadataShow full item record
AbstractMycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease, a chronic granulomatous enteritis in ruminants. Furthermore, infections of humans with MAP have been reported and a possible association with Crohn's disease and diabetes type I is currently discussed. MAP owns large sequence polymorphisms (LSPs) that were exclusively found in this mycobacteria species. The relevance of these LSPs in the pathobiology of MAP is still unclear. The mptD gene (MAP3733c) of MAP belongs to a small group of functionally uncharacterized genes, which are not present in any other sequenced mycobacteria species. mptD is part of a predicted operon (mptABCDEF), encoding a putative ATP binding cassette-transporter, located on the MAP-specific LSP14. In the present study, we generated an mptD knockout strain (MAPΔmptD) by specialized transduction. In order to investigate the potential role of mptD in the host, we performed infection experiments with macrophages. By this, we observed a significantly reduced cell number of MAPΔmptD early after infection, indicating that the mutant was hampered with respect to adaptation to the early macrophage environment. This important role of mptD was supported in mouse infection experiments where MAPΔmptD was significantly attenuated after peritoneal challenge. Metabolic profiling was performed to determine the cause for the reduced virulence and identified profound metabolic disorders especially in the lipid metabolism of MAPΔmptD. Overall our data revealed the mptD gene to be an important factor for the metabolic adaptation of MAP required for persistence in the host.
CitationThe Mycobacterium avium ssp. paratuberculosis specific mptD gene is required for maintenance of the metabolic homeostasis necessary for full virulence in mouse infections. 2014, 4:110 Front Cell Infect Microbiol
AffiliationDepartment of Infectious Diseases, Institute for Microbiology, University of Veterinary Medicine Hannover Hannover, Germany.
The following license files are associated with this item:
- Mycobacterium avium subspecies induce differential expression of pro-inflammatory mediators in a murine macrophage model: evidence for enhanced pathogenicity of Mycobacterium avium subspecies paratuberculosis.
- Authors: Basler T, Geffers R, Weiss S, Valentin-Weigand P, Goethe R
- Issue date: 2008
- A 38-kilobase pathogenicity island specific for Mycobacterium avium subsp. paratuberculosis encodes cell surface proteins expressed in the host.
- Authors: Stratmann J, Strommenger B, Goethe R, Dohmann K, Gerlach GF, Stevenson K, Li LL, Zhang Q, Kapur V, Bull TJ
- Issue date: 2004 Mar
- MAP3738c and MptD are specific tags of Mycobacterium avium subsp. paratuberculosis infection in type I diabetes mellitus.
- Authors: Cossu A, Rosu V, Paccagnini D, Cossu D, Pacifico A, Sechi LA
- Issue date: 2011 Oct
- Identification of a lineage specific zinc responsive genomic island in Mycobacterium avium ssp. paratuberculosis.
- Authors: Eckelt E, Jarek M, Frömke C, Meens J, Goethe R
- Issue date: 2014 Dec 6
- Key role for the alternative sigma factor, SigH, in the intracellular life of Mycobacterium avium subsp. paratuberculosis during macrophage stress.
- Authors: Ghosh P, Wu CW, Talaat AM
- Issue date: 2013 Jun