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dc.contributor.authorKolinko, Isabel
dc.contributor.authorLohße, Anna
dc.contributor.authorBorg, Sarah
dc.contributor.authorRaschdorf, Oliver
dc.contributor.authorJogler, Christian
dc.contributor.authorTu, Qiang
dc.contributor.authorPósfai, Mihály
dc.contributor.authorTompa, Eva
dc.contributor.authorPlitzko, Jürgen M
dc.contributor.authorBrachmann, Andreas
dc.contributor.authorWanner, Gerhard
dc.contributor.authorMüller, Rolf
dc.contributor.authorZhang, Youming
dc.contributor.authorSchüler, Dirk
dc.date.accessioned2014-11-14T14:02:12Z
dc.date.available2014-11-14T14:02:12Z
dc.date.issued2014-03
dc.identifier.citationBiosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters. 2014, 9 (3):193-7 Nat Nanotechnolen
dc.identifier.issn1748-3395
dc.identifier.pmid24561353
dc.identifier.doi10.1038/nnano.2014.13
dc.identifier.urihttp://hdl.handle.net/10033/334911
dc.description.abstractThe synthetic production of monodisperse single magnetic domain nanoparticles at ambient temperature is challenging. In nature, magnetosomes--membrane-bound magnetic nanocrystals with unprecedented magnetic properties--can be biomineralized by magnetotactic bacteria. However, these microbes are difficult to handle. Expression of the underlying biosynthetic pathway from these fastidious microorganisms within other organisms could therefore greatly expand their nanotechnological and biomedical applications. So far, this has been hindered by the structural and genetic complexity of the magnetosome organelle and insufficient knowledge of the biosynthetic functions involved. Here, we show that the ability to biomineralize highly ordered magnetic nanostructures can be transferred to a foreign recipient. Expression of a minimal set of genes from the magnetotactic bacterium Magnetospirillum gryphiswaldense resulted in magnetosome biosynthesis within the photosynthetic model organism Rhodospirillum rubrum. Our findings will enable the sustainable production of tailored magnetic nanostructures in biotechnologically relevant hosts and represent a step towards the endogenous magnetization of various organisms by synthetic biology.
dc.language.isoenen
dc.subject.meshBiotechnologyen
dc.subject.meshGene Transfer Techniquesen
dc.subject.meshGenes, Bacterialen
dc.subject.meshMagnetosomesen
dc.subject.meshMagnetospirillumen
dc.subject.meshMultigene Familyen
dc.subject.meshNanostructuresen
dc.subject.meshNanotechnologyen
dc.subject.meshRhodospirillum rubrumen
dc.titleBiosynthesis of magnetic nanostructures in a foreign organism by transfer of bacterial magnetosome gene clusters.en
dc.typeArticleen
dc.contributor.departmentLudwig-Maximilians-Universität München, Department of Biology I, Großhaderner Straße 2-4, 82152 Martinsried, Germany.en
dc.identifier.journalNature nanotechnologyen
refterms.dateFOA2018-06-13T07:27:29Z
html.description.abstractThe synthetic production of monodisperse single magnetic domain nanoparticles at ambient temperature is challenging. In nature, magnetosomes--membrane-bound magnetic nanocrystals with unprecedented magnetic properties--can be biomineralized by magnetotactic bacteria. However, these microbes are difficult to handle. Expression of the underlying biosynthetic pathway from these fastidious microorganisms within other organisms could therefore greatly expand their nanotechnological and biomedical applications. So far, this has been hindered by the structural and genetic complexity of the magnetosome organelle and insufficient knowledge of the biosynthetic functions involved. Here, we show that the ability to biomineralize highly ordered magnetic nanostructures can be transferred to a foreign recipient. Expression of a minimal set of genes from the magnetotactic bacterium Magnetospirillum gryphiswaldense resulted in magnetosome biosynthesis within the photosynthetic model organism Rhodospirillum rubrum. Our findings will enable the sustainable production of tailored magnetic nanostructures in biotechnologically relevant hosts and represent a step towards the endogenous magnetization of various organisms by synthetic biology.


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