The PqsR and RhlR transcriptional regulators determine the level of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa by producing two different pqsABCDE mRNA isoforms.
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AbstractRegulation of gene expression plays a key role in bacterial adaptability to changes in the environment. An integral part of this gene regulatory network is achieved via quorum sensing (QS) systems that coordinate bacterial responses under high cellular densities. In the nosocomial pathogen Pseudomonas aeruginosa, the 2-alkyl-4-quinolone (pqs) signaling pathway is crucial for bacterial survival under stressful conditions. Biosynthesis of the Pseudomonas quinolone signal (PQS) is dependent on the pqsABCDE operon, which is positively regulated by the LysR family regulator PqsR and repressed by the transcriptional regulator protein RhlR. However, the molecular mechanisms underlying this inhibition have remained elusive. Here, we demonstrate that not only PqsR but also RhlR activates transcription of pqsA. The latter uses an alternative transcriptional start site and induces expression of a longer transcript that forms a secondary structure in the 5' untranslated leader region. As a consequence, access of the ribosome to the Shine-Dalgarno sequence is restricted and translation efficiency reduced. We propose a model of a novel posttranscriptional regulation mechanism that fine-tunes PQS biosynthesis, thus highlighting the complexity of quorum sensing in P. aeruginosa.
CitationThe PqsR and RhlR transcriptional regulators determine the level of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa by producing two different pqsABCDE mRNA isoforms. 2014, 196 (23):4163-71 J. Bacteriol.
JournalJournal of bacteriology
The following license files are associated with this item:
- Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa.
- Authors: Wade DS, Calfee MW, Rocha ER, Ling EA, Engstrom E, Coleman JP, Pesci EC
- Issue date: 2005 Jul
- Dueling quorum sensing systems in Pseudomonas aeruginosa control the production of the Pseudomonas quinolone signal (PQS).
- Authors: McGrath S, Wade DS, Pesci EC
- Issue date: 2004 Jan 15
- PqsE functions independently of PqsR-Pseudomonas quinolone signal and enhances the rhl quorum-sensing system.
- Authors: Farrow JM 3rd, Sund ZM, Ellison ML, Wade DS, Coleman JP, Pesci EC
- Issue date: 2008 Nov
- Growth phase-differential quorum sensing regulation of anthranilate metabolism in Pseudomonas aeruginosa.
- Authors: Choi Y, Park HY, Park SJ, Park SJ, Kim SK, Ha C, Im SJ, Lee JH
- Issue date: 2011 Jul
- Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry.
- Authors: Xiao G, He J, Rahme LG
- Issue date: 2006 Jun