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AbstractVirus inactivation by chemical disinfectants is an important instrument for infection control in medical settings, but the mechanisms involved are poorly understood. In this study, we systematically investigated the effects of several antiviral treatments on hepatitis C virus (HCV) particles as model for enveloped viruses. Studies were performed with authentic cell culture-derived viruses, and the influence of chemical disinfectants, heat, and UV treatment on HCV was analyzed by the determination of infectious particles in a limiting-dilution assay, by quantitative reverse transcription-PCR, by core enzyme-linked immunosorbent assay, and by proteolytic protection assay. All different inactivation methods resulted in a loss of HCV infectivity by targeting different parts of the virus particle. Alcohols such as ethanol and 2-propanol did not affect the viral RNA genome integrity but disrupted the viral envelope membrane in a capsid protection assay. Heat and UV treatment of HCV particles resulted in direct damage of the viral genome since transfection of viral particle-associated RNA into permissive cells did not initiate RNA replication. In addition, heat incubation at 80°C disrupted the HCV envelope, rendering the viral capsid susceptible to proteolytic digest. This study demonstrated the molecular processes of viral inactivation of an enveloped virus and should facilitate the development of effective disinfection strategies in infection control not only against HCV but also against other enveloped viruses.
CitationMechanisms of methods for hepatitis C virus inactivation. 2015, 81 (5):1616-21 Appl. Environ. Microbiol.
AffiliationInstitute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research,Feodor-Lynen-Str. 7, 30625 Hannover, Germany.
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- How stable is the hepatitis C virus (HCV)? Environmental stability of HCV and its susceptibility to chemical biocides.
- Authors: Ciesek S, Friesland M, Steinmann J, Becker B, Wedemeyer H, Manns MP, Steinmann J, Pietschmann T, Steinmann E
- Issue date: 2010 Jun 15
- Inactivation and survival of hepatitis C virus on inanimate surfaces.
- Authors: Doerrbecker J, Friesland M, Ciesek S, Erichsen TJ, Mateu-Gelabert P, Steinmann J, Steinmann J, Pietschmann T, Steinmann E
- Issue date: 2011 Dec 15
- Thermal stability and inactivation of hepatitis C virus grown in cell culture.
- Authors: Song H, Li J, Shi S, Yan L, Zhuang H, Li K
- Issue date: 2010 Feb 18
- Virus inactivation mechanisms: impact of disinfectants on virus function and structural integrity.
- Authors: Wigginton KR, Pecson BM, Sigstam T, Bosshard F, Kohn T
- Issue date: 2012 Nov 6
- [Current topics on inactivation of norovirus].
- Authors: Noda M, Uema M
- Issue date: 2011