An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsJenke, Bok Hee C
Fetzer, Christian P
Stehle, Isa M
Fackelmayer, Frank O
Lipps, Hans J
MetadataShow full item record
AbstractpEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class.
CitationAn episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo. 2002, 3 (4):349-54 EMBO Rep.
AffiliationInstitute of Cell Biology, Stockumer Strasse 10, University of Witten/Herdecke, D-58448 Witten.
The following license files are associated with this item:
- The scaffold/matrix attachment region binding protein hnRNP-U (SAF-A) is directly bound to chromosomal DNA in vivo: a chemical cross-linking study.
- Authors: Göhring F, Fackelmayer FO
- Issue date: 1997 Jul 8
- A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.
- Authors: Piechaczek C, Fetzer C, Baiker A, Bode J, Lipps HJ
- Issue date: 1999 Jan 15
- Nuclear scaffold/matrix attached region modules linked to a transcription unit are sufficient for replication and maintenance of a mammalian episome.
- Authors: Jenke AC, Stehle IM, Herrmann F, Eisenberger T, Baiker A, Bode J, Fackelmayer FO, Lipps HJ
- Issue date: 2004 Aug 3
- The novel SAR-binding domain of scaffold attachment factor A (SAF-A) is a target in apoptotic nuclear breakdown.
- Authors: Göhring F, Schwab BL, Nicotera P, Leist M, Fackelmayer FO
- Issue date: 1997 Dec 15
- Specific interaction of mouse major satellite with MAR-binding protein SAF-A.
- Authors: Lobov IB, Tsutsui K, Mitchell AR, Podgornaya OI
- Issue date: 2000 Nov