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dc.contributor.authorCrull, Katja
dc.contributor.authorRohde, Manfred
dc.contributor.authorWestphal, Kathrin
dc.contributor.authorLoessner, Holger
dc.contributor.authorWolf, Kathrin
dc.contributor.authorFelipe-López, Alfonso
dc.contributor.authorHensel, Michael
dc.contributor.authorWeiss, Siegfried
dc.date.accessioned2015-04-09T09:19:51Zen
dc.date.available2015-04-09T09:19:51Zen
dc.date.issued2011-08en
dc.identifier.citationBiofilm formation by Salmonella enterica serovar Typhimurium colonizing solid tumours. 2011, 13 (8):1223-33 Cell. Microbiol.en
dc.identifier.issn1462-5822en
dc.identifier.pmid21507181en
dc.identifier.doi10.1111/j.1462-5822.2011.01612.xen
dc.identifier.urihttp://hdl.handle.net/10033/362468en
dc.description.abstractSystemic administration of Salmonella enterica serovar Typhimurium to tumour bearing mice results in preferential colonization of the tumours and retardation of tumour growth. Although the bacteria are able to invade the tumour cells in vitro, in tumours they were never detected intracellularly. Ultrastructural analysis of Salmonella-colonized tumours revealed that the bacteria had formed biofilms. Interestingly, depletion of neutrophilic granulocytes drastically reduced biofilm formation. Obviously, bacteria form biofilms in response to the immune reactions of the host. Importantly, we tested Salmonella mutants that were no longer able to form biofilms by deleting central regulators of biofilm formation. Such bacteria could be observed intracellularly in immune cells of the host or in tumour cells. Thus, tumour colonizing S. typhimurium might form biofilms as protection against phagocytosis. Since other bacteria are behaving similarly, solid murine tumours might represent a unique model to study biofilm formation in vivo.
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAntibiosisen
dc.subject.meshBiofilmsen
dc.subject.meshDisease Models, Animalen
dc.subject.meshHost-Pathogen Interactionsen
dc.subject.meshMiceen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshNeoplasmsen
dc.subject.meshNeutrophilsen
dc.subject.meshPhagocytosisen
dc.subject.meshSalmonella Infections, Animalen
dc.subject.meshSalmonella typhimuriumen
dc.titleBiofilm formation by Salmonella enterica serovar Typhimurium colonizing solid tumours.en
dc.typeArticleen
dc.identifier.journalCellular microbiologyen
refterms.dateFOA2018-06-13T00:50:37Z
html.description.abstractSystemic administration of Salmonella enterica serovar Typhimurium to tumour bearing mice results in preferential colonization of the tumours and retardation of tumour growth. Although the bacteria are able to invade the tumour cells in vitro, in tumours they were never detected intracellularly. Ultrastructural analysis of Salmonella-colonized tumours revealed that the bacteria had formed biofilms. Interestingly, depletion of neutrophilic granulocytes drastically reduced biofilm formation. Obviously, bacteria form biofilms in response to the immune reactions of the host. Importantly, we tested Salmonella mutants that were no longer able to form biofilms by deleting central regulators of biofilm formation. Such bacteria could be observed intracellularly in immune cells of the host or in tumour cells. Thus, tumour colonizing S. typhimurium might form biofilms as protection against phagocytosis. Since other bacteria are behaving similarly, solid murine tumours might represent a unique model to study biofilm formation in vivo.


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