Cryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-Å resolution.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractHepatitis C virus (HCV), a widespread human pathogen, is dependent on a highly structured 5'-untranslated region of its mRNA, referred to as internal ribosome entry site (IRES), for the translation of all of its proteins. The HCV IRES initiates translation by directly binding to the small ribosomal subunit (40S), circumventing the need for many eukaryotic translation initiation factors required for mRNA scanning. Here we present the cryo-EM structure of the human 40S ribosomal subunit in complex with the HCV IRES at 3.9 Å resolution, determined by focused refinement of an 80S ribosome-HCV IRES complex. The structure reveals the molecular details of the interactions between the IRES and the 40S, showing that expansion segment 7 (ES7) of the 18S rRNA acts as a central anchor point for the HCV IRES. The structural data rationalizes previous biochemical and genetic evidence regarding the initiation mechanism of the HCV and other related IRESs.
CitationCryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-Å resolution. 2015, 6:7646 Nat Commun
AffiliationHelmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany.
The following license files are associated with this item:
- LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex.
- Authors: Angulo J, Ulryck N, Deforges J, Chamond N, Lopez-Lastra M, Masquida B, Sargueil B
- Issue date: 2016 Feb 18
- Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit.
- Authors: Hashem Y, des Georges A, Dhote V, Langlois R, Liao HY, Grassucci RA, Pestova TV, Hellen CU, Frank J
- Issue date: 2013 Nov 28
- HCV IRES interacts with the 18S rRNA to activate the 40S ribosome for subsequent steps of translation initiation.
- Authors: Malygin AA, Kossinova OA, Shatsky IN, Karpova GG
- Issue date: 2013 Oct
- Base pairing between hepatitis C virus RNA and 18S rRNA is required for IRES-dependent translation initiation in vivo.
- Authors: Matsuda D, Mauro VP
- Issue date: 2014 Oct 28
- Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA.
- Authors: Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Bürger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CM
- Issue date: 2015 Dec 14