FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis.
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Authors
Eckelt, ElkeMeißner, Thorsten
Meens, Jochen
Laarmann, Kristin
Nerlich, Andreas
Jarek, Michael
Weiss, Siegfried
Gerlach, Gerald-F
Goethe, Ralph
Issue Date
2015
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Show full item recordAbstract
The ferric uptake regulator A (FurA) is known to be involved in iron homeostasis and stress response in many bacteria. In mycobacteria the precise role of FurA is still unclear. In the presented study, we addressed the functional role of FurA in the ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) by construction of a furA deletion strain (MAPΔfurA). RNA deep sequencing revealed that the FurA regulon consists of repressed and activated genes associated to stress response or intracellular survival. Not a single gene related to metal homeostasis was affected by furA deletion. A decisive role of FurA during intracellular survival in macrophages was shown by significantly enhanced survival of MAPΔfurA compared to the wildtype, indicating that a principal task of mycobacterial FurA is oxidative stress response regulation in macrophages. This resistance was not associated with altered survival of mice after long term infection with MAP. Our results demonstrate for the first time, that mycobacterial FurA is not involved in the regulation of iron homeostasis. However, they provide strong evidence that FurA contributes to intracellular survival as an oxidative stress sensing regulator.Citation
FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis. 2015, 6:16 Front MicrobiolAffiliation
HZI-Helmholzzentrum für InfektionsforschungJournal
Frontiers in microbiologyPubMed ID
25705205Type
ArticleLanguage
enISSN
1664-302Xae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2015.00016
Scopus Count
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