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dc.contributor.authorBielecki, Piotr
dc.contributor.authorJensen, Vanessa
dc.contributor.authorSchulze, Wiebke
dc.contributor.authorGödeke, Julia
dc.contributor.authorStrehmel, Janine
dc.contributor.authorEckweiler, Denitsa
dc.contributor.authorNicolai, Tanja
dc.contributor.authorBielecka, Agata
dc.contributor.authorWille, Thorsten
dc.contributor.authorGerlach, Roman G
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2015-10-29T11:59:02Zen
dc.date.available2015-10-29T11:59:02Zen
dc.date.issued2015-07-27en
dc.identifier.citationCross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa. 2015, 43 (13):6413-25 Nucleic Acids Res.en
dc.identifier.issn1362-4962en
dc.identifier.pmid26082498en
dc.identifier.doi10.1093/nar/gkv599en
dc.identifier.urihttp://hdl.handle.net/10033/581417en
dc.description.abstractTwo-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 TCS components. To avoid unwanted cross-talk, signaling cascades are very specific, with one sensor talking to its cognate response regulator (RR). However, cross-regulation may provide means to integrate different environmental stimuli into a harmonized output response. By applying a split luciferase complementation assay, we identified a functional interaction of two RRs of the OmpR/PhoB subfamily, namely PhoB and TctD in P. aeruginosa. Transcriptional profiling, ChIP-seq analysis and a global motif scan uncovered the regulons of the two RRs as well as a quadripartite binding motif in six promoter regions. Phosphate limitation resulted in PhoB-dependent expression of the downstream genes, whereas the presence of TctD counteracted this activation. Thus, the integration of two important environmental signals e.g. phosphate availability and the carbon source are achieved by a titration of the relative amounts of two phosphorylated RRs that inversely regulate a common subset of genes. In conclusion, our results on the PhoB and TctD mediated two-component signal transduction pathways exemplify how P. aeruginosa may exploit cross-regulation to adapt bacterial behavior to complex environments.
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshBinding Sitesen
dc.subject.meshConsensus Sequenceen
dc.subject.meshDNA-Binding Proteinsen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshLuciferasesen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshRegulonen
dc.subject.meshSignal Transductionen
dc.subject.meshTranscription, Geneticen
dc.titleCross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentHZI-Helmholzzentrum für Infektionsforschungen
dc.identifier.journalNucleic acids researchen
refterms.dateFOA2018-06-13T05:44:23Z
html.description.abstractTwo-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 TCS components. To avoid unwanted cross-talk, signaling cascades are very specific, with one sensor talking to its cognate response regulator (RR). However, cross-regulation may provide means to integrate different environmental stimuli into a harmonized output response. By applying a split luciferase complementation assay, we identified a functional interaction of two RRs of the OmpR/PhoB subfamily, namely PhoB and TctD in P. aeruginosa. Transcriptional profiling, ChIP-seq analysis and a global motif scan uncovered the regulons of the two RRs as well as a quadripartite binding motif in six promoter regions. Phosphate limitation resulted in PhoB-dependent expression of the downstream genes, whereas the presence of TctD counteracted this activation. Thus, the integration of two important environmental signals e.g. phosphate availability and the carbon source are achieved by a titration of the relative amounts of two phosphorylated RRs that inversely regulate a common subset of genes. In conclusion, our results on the PhoB and TctD mediated two-component signal transduction pathways exemplify how P. aeruginosa may exploit cross-regulation to adapt bacterial behavior to complex environments.


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